Goat Anti-Mouse IgG F(ab')2 secondary antibody (Fluorescein)
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Specification
Product Description
Goat anti-mouse IgG recognizes mouse IgG F(ab')2 fragment. This secondary antibody was purified using immunoaffinity chromatography. The antibody is conjugated with Fluorescein.
Immunogen
Mouse IgG F(ab')2 fragment
Host
Goat
Reactivity
Mouse
Form
Lyophilized
Conjugation
FITC
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads.
Conjugation Note
3.0 moles Fluorescein (FITC) per mole of IgG
Recommend Usage
FLISA (1:10000-1:50000)
Flow Cytometry (1:500-1:2500)
Immunofluorescence (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.01 M sodium phosphate, 0.15 M sodium chloride, pH 7.2 (10 mg/mL BSA, 0.01% sodium azide).
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 1 mL of deionized water (or equivalent), store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
Flow Cytometry
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Publication Reference
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Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF). I. Preparation and fractionation of labelled IgG.
D Blakeslee, M G Baines.
Journal of Immunological Methods 1976 Dec; 13(3-4):305.
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Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF). I. Preparation and fractionation of labelled IgG.
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