Sheep Anti-Human IgG (H&L) secondary antibody (Alkaline Phosphatase)
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Specification
Product Description
Sheep anti-human IgG recognizes human IgG whole molecule. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Alkaline Phosphatase.
Immunogen
Human IgG whole molecule
Host
Sheep
Reactivity
Human
Form
Liquid
Conjugation
Alkaline Phosphatase (Calf Intestine)
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities.
Isotype
IgG
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 50 mM Tris-Cl, 150 mM NaCl, 1 mM MgCl, 0.1 mM ZnCl2, pH 8.0 (50% glycerol, 0.09% sodium azide, 10 mg/mL BSA, Immunoglobulin and Protease free)
Storage Instruction
Store at 4°C. Do not Freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
Dot Blot
Enzyme Immunoassay
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Publication Reference
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Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration.
Avrameas S, Ternynck T.
Immunochemistry 1971 Dec; 8(12):1175.
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Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration.
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