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Last updated: 2019/12/1
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Sheep Anti-Human IgG (H&L) secondary antibody (Peroxidase) 

  • Catalog # : PAB10728
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  • Specification
  • Product Description:
  • Sheep anti-human IgG recognizes human IgG whole molecule. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Peroxidase.
  • Immunogen:
  • Human IgG whole molecule
  • Host:
  • Sheep
  • Reactivity:
  • Human
  • Specificity:
  • Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-sheep serum, human IgG and human serum.
  • Form:
  • Lyophilized
  • Conjugation:
  • HRP
  • Purification:
  • This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose.
  • Recommend Usage:
  • ELISA (1:150,000-1:250,000)
    Immunohistochemistry (1:500-1:2,500)
    Western Blot (1:1,000-1:10,000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (10 mg/mL BSA (immunoglobulin and protease free), 0.01% (w/v) Gentamicin Sulfate).
  • Storage Instruction:
  • Store at 4°C prior to restoration.
    After reconstitution with 1.0 mL deionized water (or equivalent), store at -20°C or below.
    Aliquot to avoid repeated freezing and thawing.
    Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
  • Note:
  • Do NOT add sodium azide! This product has been assayed against 1.0 ug of human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:6,000 to 1:36,000 of the reconstitution concentration is suggested for this product.
  • Publication Reference
  • Applications
  • Western Blot
  • Immunohistochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Western Blot
  • Immunohistochemistry
  • Enzyme-linked Immunoabsorbent Assay
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