Rabbit Anti-Bovine IgG F(ab')2 secondary antibody (Fluorescein)
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Specification
Product Description
Rabbit anti-bovine IgG recognizes bovine IgG F(ab')2 fragment. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Fluorescein.
Immunogen
Bovine IgG F(ab')2 fragment
Host
Rabbit
Reactivity
Bovine
Form
Lyophilized
Conjugation
FITC
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation Note
1.9 moles DTAF per mole of rabbit IgG
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
Lyophilized from 10 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
Flow Cytometry
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Publication Reference
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Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF). I. Preparation and fractionation of labelled IgG.
D Blakeslee, M G Baines.
Journal of Immunological Methods 1976 Dec; 13(3-4):305.
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Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF). I. Preparation and fractionation of labelled IgG.
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