SMO polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot using SMO polyclonal antibody (Cat # PAB10404) on human brain lysate. Lysate loaded at 15 ug/lane. Antibody used at 10 ug/mL (1) and 5 ug/mL (2) dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SMO.
Immunogen
A synthetic peptide corresponding tof human SMO.
Host
Rabbit
Reactivity
Human
Form
Liquid
Isotype
IgG
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western blot (1 to 5 mg/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.08% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot using SMO polyclonal antibody (Cat # PAB10404) on human brain lysate. Lysate loaded at 15 ug/lane. Antibody used at 10 ug/mL (1) and 5 ug/mL (2) dilution.Western Blot
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Gene Info — SMO
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Interactome
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Pathway
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Disease
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Publication Reference
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Fused-Costal2 protein complex regulates Hedgehog-induced Smo phosphorylation and cell-surface accumulation.
Liu Y, Cao X, Jiang J, Jia J.
Genes & Development 2007 Aug; 21(15):1949.
Application：IF, WB, Insect, S2 cells.
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Activating Smoothened mutations in sporadic basal-cell carcinoma.
Xie J, Murone M, Luoh SM, Ryan A, Gu Q, Zhang C, Bonifas JM, Lam CW, Hynes M, Goddard A, Rosenthal A, Epstein EH Jr, de Sauvage FJ.
Nature 1998 Jan; 391(6662):90.
Application：IHC, Mouse, Mouse skins.
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The tumour-suppressor gene patched encodes a candidate receptor for Sonic hedgehog.
Stone DM, Hynes M, Armanini M, Swanson TA, Gu Q, Johnson RL, Scott MP, Pennica D, Goddard A, Phillips H, Noll M, Hooper JE, de Sauvage F, Rosenthal A.
Nature 1996 Nov; 384(6605):129.
Application：IF, IP, Monkey, COS-7 cells.
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Fused-Costal2 protein complex regulates Hedgehog-induced Smo phosphorylation and cell-surface accumulation.
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