Phosphothreonine polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Phospho Threonine polyclonal antibody (Cat # PAB10352) is shown to detect phosphorylated proteins present in mouse spleen cell lysates. Left Lane: extract of spleen from mouse treated with vanadium. Right lane: extract of spleen from untreated mouse.
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Specification
Product Description
Rabbit polyclonal antibody raised against Phosphothreonine.
Immunogen
Phosphothreonine conjugated with KLH.
Host
Rabbit
Specificity
Reactivity is specific to phosphothreonine and minimal cross reactivity is observed against phosphotyrosine or phosphoserine.
Form
Liquid
Quality Control Testing
Antibody Reactive Against PhosphoThreonine.
Recommend Usage
ELISA (0.5 ug/mL)
Western Blot (2.0 ug/mL)
Immunoprecipitation (10.0 ug/mg sample)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Phospho Threonine polyclonal antibody (Cat # PAB10352) is shown to detect phosphorylated proteins present in mouse spleen cell lysates. Left Lane: extract of spleen from mouse treated with vanadium. Right lane: extract of spleen from untreated mouse.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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