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Last updated: 2017/4/30
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MYC-tag polyclonal antibody

  • Catalog # : PAB10345
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of MYC-tag.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to amino acids 410-419 of human MYC-tag.
  • Sequence:
  • EQKLISEEDL
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • This affinity purified antibody is directed against human c-Myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. This antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:135000)
    Western Blot (1:500-1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • MYC-tag polyclonal antibody (Cat # PAB10345) detects ~60 kDa amino terminal linked MYC-tagged recombinant protein by western blot (arrowhead).
    Lane 1 contains ~35 ug of lysate from control 293T cells.
    Lane 2 contains ~35 ug of lysate from 293T cells overexpressing an N-terminal linked recombinant protein.
    Amino terminal linked MYC recombinant protein was the gift of Brian Conti, University of North Carolina, Chapel Hill, NC.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • MYC-tag polyclonal antibody (Cat # PAB10345) detects ~ 100 kDa carboxy terminal linked MYC-tagged recombinant protein present in ~35 ug of lysate by western blot (arrowhead).
    Carboxy terminal linked MYC recombinant protein was the gift of Zhongsheng You, Salk Institute, LaJolla, CA.
  • Immunohistochemistry
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4609
  • Gene Name:
  • MYC
  • Gene Alias:
  • bHLHe39,c-Myc
  • Gene Description:
  • v-myc myelocytomatosis viral oncogene homolog (avian)
  • Gene Summary:
  • The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq
  • Other Designations:
  • avian myelocytomatosis viral oncogene homolog,myc proto-oncogene protein,v-myc avian myelocytomatosis viral oncogene homolog
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