Rabbit polyclonal antibody raised against synthetic peptide of MYC-tag.
A synthetic peptide (conjugated with KLH) corresponding to amino acids 410-419 of human MYC-tag.
This affinity purified antibody is directed against human c-Myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. This antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
ELISA (1:135000) Western Blot (1:500-1:5000) The optimal working dilution should be determined by the end user.
In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
MYC-tag polyclonal antibody (Cat # PAB10345) detects ~60 kDa amino terminal linked MYC-tagged recombinant protein by western blot (arrowhead). Lane 1 contains ~35 ug of lysate from control 293T cells. Lane 2 contains ~35 ug of lysate from 293T cells overexpressing an N-terminal linked recombinant protein. Amino terminal linked MYC recombinant protein was the gift of Brian Conti, University of North Carolina, Chapel Hill, NC.
Western Blot (Recombinant protein)
MYC-tag polyclonal antibody (Cat # PAB10345) detects ~ 100 kDa carboxy terminal linked MYC-tagged recombinant protein present in ~35 ug of lysate by western blot (arrowhead). Carboxy terminal linked MYC recombinant protein was the gift of Zhongsheng You, Salk Institute, LaJolla, CA.
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq