MBP polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant MBP (Maltose Binding Protein).
Immunogen
Recombinant protein corresponding to MBP (Maltose Binding Protein).
Host
Rabbit
Specificity
This polyclonal anti-MBP epitope tag antibody detects over-expressed proteins containing the MBP epitope tag.
Form
Lyophilized
Isotype
IgG
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:4000-1:16000)
Western Blot (1:3500)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
MBP polyclonal antibody (Cat # PAB10344) detects MBP-tagged recombinant proteins by western blot.
MBP epitope tag polyclonal antibody at 0.5-1.0 ug/mL was used to detect 1.0 ug of recombinant protein containing the MBP epitope tag.
A 4-20% gradient gel was used to separate the protein by SDS-PAGE.
The protein was transferred to nitrocellulose using standard methods.
After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1 : 2500 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature.
LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Design of peptides as inhibitors of human papillomavirus 16 transcriptional regulator E1-E2.
Kantang W, Chunsrivirot S, Muangsin N, Poovorawan Y, Krusong K.
Chemical Biology & Drug Design 2016 Jun; 88(4):475.
Application:ELISA, WB, Recombinant protein.
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Design of peptides as inhibitors of human papillomavirus 16 transcriptional regulator E1-E2.
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