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Last updated: 2016/12/4
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MBP polyclonal antibody

  • Catalog # : PAB10344
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against recombinant MBP (Maltose Binding Protein).
  • Immunogen:
  • Recombinant protein corresponding to MBP (Maltose Binding Protein).
  • Host:
  • Rabbit
  • Specificity:
  • This polyclonal anti-MBP epitope tag antibody detects over-expressed proteins containing the MBP epitope tag.
  • Form:
  • Lyophilized
  • Isotype:
  • IgG
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:4000-1:16000)
    Western Blot (1:3500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • MBP polyclonal antibody (Cat # PAB10344) detects MBP-tagged recombinant proteins by western blot.
    MBP epitope tag polyclonal antibody at 0.5-1.0 ug/mL was used to detect 1.0 ug of recombinant protein containing the MBP epitope tag.
    A 4-20% gradient gel was used to separate the protein by SDS-PAGE.
    The protein was transferred to nitrocellulose using standard methods.
    After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1 : 2500 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature.
    LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.
  • ELISA
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • ELISA
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