GFP polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot of GFP protein detected with GFP polyclonal antibody (Cat # PAB10342).
Wild type GFP (0.1 ug) was used to spike 30 ug of a HeLa whole cell lysate.
This antibody detects a 27 KDa band corresponding to the epitope tag GFP.
A 4-20% Tris-Glycine gradient gel was used for SDS-PAGE.
The protein was transferred to nitrocellulose using standard methods.
After blocking with 5% BLOTTO in PBS, the membrane was probed overnight at 4°C with the primary antibody diluted in 5% BLOTTO to 1 : 1,000, followed by washes and reaction with a 1 : 10,000 dilution of IRDye® 800 conjugated Goat-a-Rabbit IgG [H&L] MX10.
IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.
-
Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant GFP.
Immunogen
Recombinant protein corresponding to full length jellyfish Aequorea victoria GFP.
Host
Rabbit
Reactivity
Jellyfishes
Specificity
No reaction was observed against human, Mouse or Rat serum proteins.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:24000-1:100000)
Western Blot (1:500-1:5000)
Immunohistochemistry (1:200-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot of GFP protein detected with GFP polyclonal antibody (Cat # PAB10342).
Wild type GFP (0.1 ug) was used to spike 30 ug of a HeLa whole cell lysate.
This antibody detects a 27 KDa band corresponding to the epitope tag GFP.
A 4-20% Tris-Glycine gradient gel was used for SDS-PAGE.
The protein was transferred to nitrocellulose using standard methods.
After blocking with 5% BLOTTO in PBS, the membrane was probed overnight at 4°C with the primary antibody diluted in 5% BLOTTO to 1 : 1,000, followed by washes and reaction with a 1 : 10,000 dilution of IRDye® 800 conjugated Goat-a-Rabbit IgG [H&L] MX10.
IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com