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Last updated: 2017/12/17

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GFP polyclonal antibody

  • Catalog # : PAB10342
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against full length recombinant GFP.
  • Immunogen:
  • Recombinant protein corresponding to full length jellyfish Aequorea victoria GFP.
  • Host:
  • Rabbit
  • Reactivity:
  • Jellyfishes
  • Specificity:
  • No reaction was observed against human, Mouse or Rat serum proteins.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:24000-1:100000)
    Western Blot (1:500-1:5000)
    Immunohistochemistry (1:200-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot of GFP protein detected with GFP polyclonal antibody (Cat # PAB10342).
    Wild type GFP (0.1 ug) was used to spike 30 ug of a HeLa whole cell lysate.
    This antibody detects a 27 KDa band corresponding to the epitope tag GFP.
    A 4-20% Tris-Glycine gradient gel was used for SDS-PAGE.
    The protein was transferred to nitrocellulose using standard methods.
    After blocking with 5% BLOTTO in PBS, the membrane was probed overnight at 4°C with the primary antibody diluted in 5% BLOTTO to 1 : 1,000, followed by washes and reaction with a 1 : 10,000 dilution of IRDye® 800 conjugated Goat-a-Rabbit IgG [H&L] MX10.
    IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDye is a trademark of LI-COR, Inc.
  • Immunohistochemistry
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry
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