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Last updated: 2017/7/16
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GFP polyclonal antibody

  • Catalog # : PAB10341
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against full length recombinant GFP.
  • Immunogen:
  • Recombinant protein corresponding to full length jellyfish Aequorea victoria GFP.
  • Host:
  • Goat
  • Reactivity:
  • Jellyfishes
  • Specificity:
  • No reaction was observed against human, Mouse or Rat serum proteins.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:40000)
    Western Blot (1:400-1:2000)
    Immunohistochemistry (1:200-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot of GFP recombinant protein detected with GFP polyclonal antibody (Cat # PAB10341).
    Lane 1 shows detection of a 33 KDa band corresponding to a GFP containing recombinant protein (arrowhead) expressed in HeLa cells.
    Lane 2 shows no staining of a mock transfected HeLa cell lysate.
    A 4-12% Bis-Tris gradient gel was used for SDS-PAGE.
    The protein was transferred to nitrocellulose using standard methods.
    After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/mL for 1 h at room temperature followed by washes and reaction with a 1 : 2,500 dilution of IRDye® 800 conjugated Donkey-a-Goat IgG [H&L] MX7.
    The IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDye is a trademark of LI-COR, Inc.
  • Immunohistochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence microscopy of GFP polyclonal antibody (Cat # PAB10341) at a 1 : 1,000 dilution detects tau-GFP in cell bodies (large arrowhead) and axons of motorneurons (arrow) and interneurons (small arrowhead) in Drosophila melanogaster late stage embryonic central nervous system.
    AlexaFluor 488 conjugated anti-Goat antibody was used for detection at 1 : 300.
    Panel A shows a lateral view (ventral left) and Panels B and C show ventral views of whole mount embryos at 63x magnification (plus 2x digital zoom).
    Personal Communication, Helmata Mistry, Washington University School of Medicine, St. Louis, MO.
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry
  • ELISA
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