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Last updated: 2017/9/17
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DYKDDDDK tag polyclonal antibody

  • Catalog # : PAB10340
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptides of DYKDDDDK tag.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to DYKDDDDK tag.
  • Host:
  • Rabbit
  • Specificity:
  • This antibody reacts with FLAG conjugated proteins.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:90000-1:250000)
    Western Blot (1:2000-1:10000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • DYKDDDDK tag polyclonal antibody (Cat # PAB10340) to detect FLAG™ conjugated proteins detects both C terminal linked and N terminal linked FLAG™ tagged recombinant proteins by western blot.
    This antibody was used at a dilution of 1 : 2,500 to detect 1.0 ug of recombinant protein containing either the FLAG™ epitope tag linked at the carboxy (C) or the amino (N) terminus of the recombinant protein.
    A 4-20% gradient gel was used to resolve the protein by SDS-PAGE.
    The protein was transferred to nitrocellulose using standard methods.
    After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 30 min at room temperature.
    LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.
  • ELISA
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • ELISA
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