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Last updated: 2017/3/26
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RELA (phospho S536) polyclonal antibody

  • Catalog # : PAB10338
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of RELA.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S536 of human RELA.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody is specific to human p65 (RelA) protein phosphorylated at S536.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:1000-1:6000)
    Western Blot (1:200-1:2000)
    Immunohistochemistry (5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot using RELA (phospho S536) polyclonal antibody (Cat # PAB10338) shows detection of RELA phosphorylated at S536.
    The control blot (left lane) contains 100 ng of purified RELA-GST fusion protein.
    The band is seen (right lane) when this protein is phosphorylated at S536 by IKKbeta.
    Personal Communication. J. Brady, NCI, Bethesda, MD.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of RELA (phospho S536) polyclonal antibody (Cat # PAB10338) was used at 5.0 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
    This image shows moderate positive staining of human kidney distal tubules and collecting ducts.
    Tissue was formalin-fixed and paraffin embedded.
    The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
    Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
  • ELISA
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5970
  • Gene Name:
  • RELA
  • Gene Alias:
  • MGC131774,NFKB3,p65
  • Gene Description:
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • Gene Summary:
  • NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM
  • Other Designations:
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells 3,v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65)),v-rel reticuloendotheliosis viral oncogene homolog
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