RELA (phospho S536) polyclonal antibody
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More Files
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Western Blot (Recombinant protein)
Western blot using RELA (phospho S536) polyclonal antibody (Cat # PAB10338) shows detection of RELA phosphorylated at S536.
The control blot (left lane) contains 100 ng of purified RELA-GST fusion protein.
The band is seen (right lane) when this protein is phosphorylated at S536 by IKKbeta.
Personal Communication. J. Brady, NCI, Bethesda, MD.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of RELA (phospho S536) polyclonal antibody (Cat # PAB10338) was used at 5.0 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderate positive staining of human kidney distal tubules and collecting ducts.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of RELA.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S536 of human RELA.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to human p65 (RelA) protein phosphorylated at S536.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:1000-1:6000)
Western Blot (1:200-1:2000)
Immunohistochemistry (5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot using RELA (phospho S536) polyclonal antibody (Cat # PAB10338) shows detection of RELA phosphorylated at S536.
The control blot (left lane) contains 100 ng of purified RELA-GST fusion protein.
The band is seen (right lane) when this protein is phosphorylated at S536 by IKKbeta.
Personal Communication. J. Brady, NCI, Bethesda, MD.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of RELA (phospho S536) polyclonal antibody (Cat # PAB10338) was used at 5.0 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderate positive staining of human kidney distal tubules and collecting ducts.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RELA
Entrez GeneID
5970Protein Accession#
Q04206Gene Name
RELA
Gene Alias
MGC131774, NFKB3, p65
Gene Description
v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Omim ID
164014Gene Ontology
HyperlinkGene Summary
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM
Other Designations
nuclear factor of kappa light polypeptide gene enhancer in B-cells 3|v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65))|v-rel reticuloendotheliosis viral oncogene homolog
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Interactome
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Pathway
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Disease
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Publication Reference
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A novel NF-kappaB pathway involving IKKbeta and p65/RelA Ser-536 phosphorylation results in p53 Inhibition in the absence of NF-kappaB transcriptional activity.
Jeong SJ, Pise-Masison CA, Radonovich MF, Park HU, Brady JN.
The Journal of Biological Chemistry 2005 Mar; 280(11):10326.
Application：WB-Tr, Mouse, C81, MEFs.
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Control of oncogenesis and cancer therapy resistance by the transcription factor NF-kappaB.
Baldwin AS.
The Journal of Clinical Investigation 2001 Feb; 107(3):241.
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Series introduction: the transcription factor NF-kappaB and human disease.
Baldwin AS Jr.
J Clin Invest 2001 Jan; 107(1):3.
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A novel NF-kappaB pathway involving IKKbeta and p65/RelA Ser-536 phosphorylation results in p53 Inhibition in the absence of NF-kappaB transcriptional activity.
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