MTOR (phospho S2448) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Immunoblotting of MTOR (phospho S2448) polyclonal antibody (Cat # PAB10335) is shown to detect a 250 kDa band (indicated) corresponding to phosphorylated human MTOR present in a 293T whole cell lysates.
Cells were serum-starved for 24 hours prior to harvest.
Approximately 20 ug of lysate was loaded per lane for SDS-PAGE.
Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/mL) for 20 min prior to harvest.
Follow reaction of antibody with a 1 : 2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of Phospho-MTOR S2448 polyclonal antibody (Cat # PAB10335) was used at 5 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderate staining of proximal convoluted tubules of the kidney.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of MTOR.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S2448 of human MTOR.
Host
Rabbit
Reactivity
Human
Specificity
Reactivity occurs with phosphorylated mTOR from human derived tissues and cells. Reactivity with mouse and rat is suggested based on protein sequence homologies.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:15000-1:65000)
Western Blot (1:500-1:2000)
Immunohistochemistry (5.0 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Immunoblotting of MTOR (phospho S2448) polyclonal antibody (Cat # PAB10335) is shown to detect a 250 kDa band (indicated) corresponding to phosphorylated human MTOR present in a 293T whole cell lysates.
Cells were serum-starved for 24 hours prior to harvest.
Approximately 20 ug of lysate was loaded per lane for SDS-PAGE.
Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/mL) for 20 min prior to harvest.
Follow reaction of antibody with a 1 : 2000 dilution of HRP Goat-a-Rabbit IgG for visualization.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of Phospho-MTOR S2448 polyclonal antibody (Cat # PAB10335) was used at 5 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderate staining of proximal convoluted tubules of the kidney.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MTOR
Entrez GeneID
2475Protein Accession#
P42345Gene Name
MTOR
Gene Alias
FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Gene Description
mechanistic target of rapamycin
Omim ID
601231Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq
Other Designations
FK506 binding protein 12-rapamycin associated protein 1|FK506 binding protein 12-rapamycin associated protein 2|FK506-binding protein 12-rapamycin complex-associated protein 1|FKBP-rapamycin associated protein|FKBP12-rapamycin complex-associated protein 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Phospholipase D confers rapamycin resistance in human breast cancer cells.
Chen Y, Zheng Y, Foster DA.
Oncogene 2003 Jun; 22(25):3937.
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Stimulation of signal transducer and activator of transcription-1 (STAT1)-dependent gene transcription by lipopolysaccharide and interferon-gamma is regulated by mammalian target of rapamycin.
Kristof AS, Marks-Konczalik J, Billings E, Moss J.
The Journal of Biological Chemistry 2003 Sep; 278(36):33637.
Application：WB-Ce, Human, A549 cells.
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The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif.
Nojima H, Tokunaga C, Eguchi S, Oshiro N, Hidayat S, Yoshino K, Hara K, Tanaka N, Avruch J, Yonezawa K.
The Journal of Biological Chemistry 2003 May; 278(18):15461.
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Phospholipase D confers rapamycin resistance in human breast cancer cells.
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