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Last updated: 2016/10/23
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MTOR (phospho S2448) polyclonal antibody

  • Catalog # : PAB10335
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of MTOR.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S2448 of human MTOR.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • Reactivity occurs with phosphorylated mTOR from human derived tissues and cells. Reactivity with mouse and rat is suggested based on protein sequence homologies.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:15000-1:65000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (5.0 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Immunoblotting of MTOR (phospho S2448) polyclonal antibody (Cat # PAB10335) is shown to detect a 250 kDa band (indicated) corresponding to phosphorylated human MTOR present in a 293T whole cell lysates.
    Cells were serum-starved for 24 hours prior to harvest.
    Approximately 20 ug of lysate was loaded per lane for SDS-PAGE.
    Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/mL) for 20 min prior to harvest.
    Follow reaction of antibody with a 1 : 2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of Phospho-MTOR S2448 polyclonal antibody (Cat # PAB10335) was used at 5 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
    This image shows moderate staining of proximal convoluted tubules of the kidney.
    Tissue was formalin-fixed and paraffin embedded.
    The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 2475
  • Gene Name:
  • MTOR
  • Gene Alias:
  • Gene Description:
  • mechanistic target of rapamycin
  • Gene Summary:
  • The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq
  • Other Designations:
  • FK506 binding protein 12-rapamycin associated protein 1,FK506 binding protein 12-rapamycin associated protein 2,FK506-binding protein 12-rapamycin complex-associated protein 1,FKBP-rapamycin associated protein,FKBP12-rapamycin complex-associated protein 1
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