Rabbit polyclonal antibody raised against synthetic phosphopeptide of Mdm2.
Synthetic phosphopeptide corresponding to residues surrounding S185 of mouse Mdm2.
Reactivity occurs against Mouse MDM2 pS185 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated mouse MDM2 is minimal by ELISA and western blot.
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
ELISA (1:3000-1:12000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunoblotting of Mdm2 (phospho S185) polyclonal antibody (Cat # PAB10334) is shown to detect a 102 kDa band (arrow) corresponding to phosphorylated mouse Mdm2 present in a 293T whole cell lysate. Cells were serum-starved for 24 hours prior to harvest. Approximately 20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/mL) for 20 min prior to harvest. Follow reaction of antibody with a 1 : 2000 dilution of HRP Goat-a-Rabbit IgG for visualization.