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Last updated: 2016/11/27
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Mdm2 (phospho S185) polyclonal antibody

  • Catalog # : PAB10334
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of Mdm2.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S185 of mouse Mdm2.
  • Host:
  • Rabbit
  • Reactivity:
  • Mouse
  • Specificity:
  • Reactivity occurs against Mouse MDM2 pS185 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated mouse MDM2 is minimal by ELISA and western blot.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:3000-1:12000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Immunoblotting of Mdm2 (phospho S185) polyclonal antibody (Cat # PAB10334) is shown to detect a 102 kDa band (arrow) corresponding to phosphorylated mouse Mdm2 present in a 293T whole cell lysate.
    Cells were serum-starved for 24 hours prior to harvest.
    Approximately 20 ug of lysate was loaded per lane for SDS-PAGE.
    Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/mL) for 20 min prior to harvest.
    Follow reaction of antibody with a 1 : 2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Gene Name:
  • Mdm2
  • Gene Alias:
  • 1700007J15Rik,AA415488,Mdm-2
  • Gene Description:
  • transformed mouse 3T3 cell double minute 2
  • Other Designations:
  • OTTMUSP00000022916
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