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Last updated: 2016/12/4
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MLF1IP (phospho T78) polyclonal antibody

  • Catalog # : PAB10333
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of MLF1IP.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding T78 of human MLF1IP.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Chimpanzee, Dog, Human, Mouse, Rat
  • Specificity:
  • This antibody is specific to human MLF1IP protein phosphorylated at Thr78.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:5000-1:25000)
    Western Blot (1:500-1:2000)
    Immunocytochemistry (1:100-1:500)
    Immunohistochemistry (20 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot using MLF1IP (phospho T78) polyclonal antibody (Cat # PAB10333) shows detection of MLF1IP phosphorylated at Thr78.
    HeLa cells were co-infected with the indicated adenoviruses expressing GFP-tagged Plk1 or PBIP1.
    Blots were probed with the MLF1IP (phospho T78) polyclonal antibody (Cat # PAB10333), stripped, and then reprobed with anti-GFP antibody (Kang & Park, et al., 2006).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of MLF1IP (phospho T78) polyclonal antibody (Cat # PAB10333) was used at 20 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
    This image shows moderately positive staining of mitotic cells in colon adenocarcinoma at 60X.
    Tissue was formalin-fixed and paraffin embedded.
    The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
    Personal Communi-cation, Tina Roush, Life Span Biosciences, Seattle, WA.
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_078905;Q71F23
  • Gene Name:
  • MLF1IP
  • Gene Alias:
  • CENP-50,CENP-U,CENP-U(50),CENP50,CENPU,FLJ23468,KLIP1,PBIP1
  • Gene Description:
  • MLF1 interacting protein
  • Gene Summary:
  • The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). MLF1IP, or CENPU, is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM
  • Other Designations:
  • KSHV latent nuclear antigen interacting protein 1,centromere protein U,polo-box-interacting protein 1
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