Products

Western Blot (Transfected lysate)

Western blot using MLF1IP (phospho T78) polyclonal antibody (Cat # PAB10333) shows detection of MLF1IP phosphorylated at Thr78.
HeLa cells were co-infected with the indicated adenoviruses expressing GFP-tagged Plk1 or PBIP1.
Blots were probed with the MLF1IP (phospho T78) polyclonal antibody (Cat # PAB10333), stripped, and then reprobed with anti-GFP antibody (Kang & Park, et al., 2006).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemistry of MLF1IP (phospho T78) polyclonal antibody (Cat # PAB10333) was used at 20 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderately positive staining of mitotic cells in colon adenocarcinoma at 60X.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal Communi-cation, Tina Roush, Life Span Biosciences, Seattle, WA.

Enzyme-linked Immunoabsorbent Assay

Entrez GeneID

79682

Protein Accession#

NP_078905;Q71F23

Gene Name

MLF1IP

Gene Alias

CENP-50, CENP-U, CENP-U(50), CENP50, CENPU, FLJ23468, KLIP1, PBIP1

Gene Description

MLF1 interacting protein

Omim ID

611511

Gene Ontology

Hyperlink

Gene Summary

The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). MLF1IP, or CENPU, is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM

Other Designations

KSHV latent nuclear antigen interacting protein 1|centromere protein U|polo-box-interacting protein 1