Rabbit polyclonal antibody raised against synthetic phosphopeptide of MARK2.
Synthetic phosphopeptide corresponding to residues surrounding T595 of human MARK2.
Bovine, Dog, Human, Mouse, Rat
Reactivity occurs against human MARK2 pT595 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human MARK2 is minimal by ELISA. Expect at least partial reactivity with other isoforms of MARK. The immunogen sequence is present in all forms identified to date and localizes to T595 on MARK2, T587 on MARK3, T591 on MARK1 and T568 on MARK4.
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
ELISA (1:1000-1:3000) Western Blot (1:250-1:1000) The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot using MARK2 (phospho T595) polyclonal antibody (Cat # PAB10332) shows detection of a band at ~82 kDa corresponding to phosphorylated MARK2 (arrowhead). HEK293 cells were transfected with pCMV-3 x Flag-MARK2 plus pCMV-Flag-PKC-zeta (lane 3) or pCMV-3 x Flag-MARK2 only (lane 4). Total cell lysates were run in a 4-12% Nupage SDS-gel and probed with MARK2 (phospho T595) polyclonal antibody (Cat # PAB10332). An untransfected HEK293 cell lysate was used as a negative control (lane 2). MW markers are shown for size comparison (lane 1). PKC-zeta appears to be involved in the phosphorylation of T595 on MARK2 as increased amounts of phospho- specific staining are observed in lysates from cells transfected with both MARK2 and PKC-zeta. Personnel Communication, S. Shaw, NCI, Center for Cancer Research.
This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
ELKL motif kinase 1,Ser/Thr protein kinase PAR-1B,protein-serine/threonine kinase,serine/threonine kinase