Il7r (phospho Y449) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Il7r.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Y449 of mouse Il7r.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to mouse IL-7 protein phosphorylated at Y449.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:100000)
Western Blot (1:1000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — Il7r
Entrez GeneID
16197Protein Accession#
P31749;P16872Gene Name
Il7r
Gene Alias
CD127, IL-7Ralpha, MGC107557
Gene Description
interleukin 7 receptor
Gene Ontology
HyperlinkOther Designations
IL-7 receptor alpha chain|interleukin 7 receptor alpha chain
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Publication Reference
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Impaired CD8 T cell memory and CD4 T cell primary responses in IL-7R alpha mutant mice.
Osborne LC, Dhanji S, Snow JW, Priatel JJ, Ma MC, Miners MJ, Teh HS, Goldsmith MA, Abraham N.
The Journal of Experimental Medicine 2007 Mar; 204(3):619.
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Chemokine-guided CD4+ T cell help enhances generation of IL-6RalphahighIL-7Ralpha high prememory CD8+ T cells.
Castellino F, Germain RN.
The Journal of Immunology 2007 Jan; 178(2):778.
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Human CD4+ regulatory T cells express lower levels of the IL-7 receptor alpha chain (CD127), allowing consistent identification and sorting of live cells.
Hartigan-O'Connor DJ, Poon C, Sinclair E, McCune JM.
Journal of Immunological Methods 2007 Jan; 319(1-2):41.
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Impaired CD8 T cell memory and CD4 T cell primary responses in IL-7R alpha mutant mice.
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