GYS1 (phospho S640) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Immunoblotting of GYS1 (phospho S640) polyclonal antibody (Cat # PAB10328) to human GYS1 at pS640 was used at a 1 : 1000 dilution to detect human muscle GYS1 by Western blot.
Approximately 12 uL of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE.
Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 uM (lanes 2, 3 and 4 respectively) for 15' or CLA treated at 4 nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45'.
After washing, a 1 : 5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of GYS1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S640 of human GYS1.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This phospho specific polyclonal antibody is specific to phosphorylated pS640 of human muscle glycogen synthase. Reactivity with non-phosphorylated human muscle glycogen synthase is less than 1% by ELISA.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Sandwich ELISA (1:30000-1:150000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Immunoblotting of GYS1 (phospho S640) polyclonal antibody (Cat # PAB10328) to human GYS1 at pS640 was used at a 1 : 1000 dilution to detect human muscle GYS1 by Western blot.
Approximately 12 uL of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE.
Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 uM (lanes 2, 3 and 4 respectively) for 15' or CLA treated at 4 nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45'.
After washing, a 1 : 5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — GYS1
Entrez GeneID
2997Gene Name
GYS1
Gene Alias
GSY, GYS
Gene Description
glycogen synthase 1 (muscle)
Omim ID
138570Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene
Other Designations
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Interactome
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Pathway
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Disease
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