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Last updated: 2017/2/19
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GYS1 (phospho S640) polyclonal antibody

  • Catalog # : PAB10328
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of GYS1.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S640 of human GYS1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • This phospho specific polyclonal antibody is specific to phosphorylated pS640 of human muscle glycogen synthase. Reactivity with non-phosphorylated human muscle glycogen synthase is less than 1% by ELISA.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Sandwich ELISA (1:30000-1:150000)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Immunoblotting of GYS1 (phospho S640) polyclonal antibody (Cat # PAB10328) to human GYS1 at pS640 was used at a 1 : 1000 dilution to detect human muscle GYS1 by Western blot.
    Approximately 12 uL of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE.
    Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 uM (lanes 2, 3 and 4 respectively) for 15' or CLA treated at 4 nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45'.
    After washing, a 1 : 5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system.
  • Immunohistochemistry
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 2997
  • Gene Name:
  • GYS1
  • Gene Alias:
  • GSY,GYS
  • Gene Description:
  • glycogen synthase 1 (muscle)
  • Gene Summary:
  • The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene
  • Other Designations:
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