Rabbit polyclonal antibody raised against synthetic phosphopeptide of GYS1.
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S640 of human GYS1.
This phospho specific polyclonal antibody is specific to phosphorylated pS640 of human muscle glycogen synthase. Reactivity with non-phosphorylated human muscle glycogen synthase is less than 1% by ELISA.
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
Sandwich ELISA (1:30000-1:150000) Western Blot (1:1000) The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunoblotting of GYS1 (phospho S640) polyclonal antibody (Cat # PAB10328) to human GYS1 at pS640 was used at a 1 : 1000 dilution to detect human muscle GYS1 by Western blot. Approximately 12 uL of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 uM (lanes 2, 3 and 4 respectively) for 15' or CLA treated at 4 nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45'. After washing, a 1 : 5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system.
The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene