Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDC16.
Synthetic phosphopeptide corresponding to residues surrounding T580 of human CDC16.
This antibody is specific to phosphorylated human APC6 protein at the pT580 residue. This antibody is expected to react with all isoforms of this protein. Minimal reactivity is expected with the non-phosphorylated form of the protein.
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
ELISA (1:1000-1:10000) Western Blot (1:500-1:3000) The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot using CDC16 (phospho T580) polyclonal antibody (Cat # PAB10325) shows detectionof a band ~ 72 KDa corresponding to phosphorylated human CDC16 (arrowhead lane 1). Lane 1 -nocodazole treated HeLa whole cell lysate. Lane 2 -Reactivity is not seen in lysates from asynchronous HeLa whole cell cultures. Each lane contains approximately 35 ug of lysates, separated by 4-20% SDS-PAGE Tris-HEPES and then transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1 : 1,000 overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
This gene encodes a component protein of the APC complex, which is composed of eight proteins and functions as a protein ubiquitin ligase. The APC complex is a cyclin degradation system that governs exit from mitosis. Each component protein of the APC complex is highly conserved among eukaryotic organisms. This protein and two other APC complex proteins, CDC23 and CDC27, contain a tetratricopeptide repeat (TPR), a protein domain that may be involved in protein-protein interaction. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq