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Last updated: 2017/7/23
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PRKDC (phospho T2609) polyclonal antibody

  • Catalog # : PAB10324
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of PRKDC.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding T2609 of human PRKDC.
  • Host:
  • Rabbit
  • Reactivity:
  • Chicken, Chimpanzee, Dog, Horse, Human, Rat
  • Specificity:
  • This phospho-specific polyclonal antibody reacts with phosphorylated pT2609 of human DNA PKcs. Reactivity with non-phosphorylated human DNA PKcs is minimal.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:300000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using PRKDC (phospho T2609) polyclonal antibody (Cat # PAB10324) shows detection of a 460 KDa band corresponding to human PRKDC in various preparations.
    Lane 1 : Fus1 untreated, Lane 2 : Fus1 IR (20Gy, 4h), Lane 3 : Fus1 PRKDC inhibitor + IR, Lane 4 : MO59J (DNAPK-) untreated, Lane 5 : MO59J IR, Lane 6 : Fus1 untreated, Lane 7 : Fus1 IR (20Gy, 4h), Lane 8 : Fus1 DNAPK inhibitor + IR, Lane 9 : MO59J untreated, Lane 10 : MO59J IR.
    Lanes 1~5 are nuclear extract whereas lanes 6~10 are whole cell lysates.
    MO59J is a cell line that lacks PRKDC.
    FUS1 is the matched cell line complemented with a chromosomal fragment containing the PRKDC gene.
    Approximately 20 $micro;g of lysate was run on SDS-PAGE and transferred onto nitrocellulose, followed by reaction with a 1 : 1,000 dilution of PRKDC (phospho T2609) polyclonal antibody (Cat # PAB10324).
    Detection occurred using a 1 : 5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature.
    A che
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5591
  • Protein Accession#:
  • P78527;NP_008835
  • Gene Name:
  • PRKDC
  • Gene Alias:
  • DNA-PKcs,DNAPK,DNPK1,HYRC,HYRC1,XRCC7,p350
  • Gene Description:
  • protein kinase, DNA-activated, catalytic polypeptide
  • Gene Summary:
  • The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.[supplied by OMIM
  • Other Designations:
  • hyper-radiosensitivity of murine scid mutation, complementing 1
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