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Last updated: 2017/11/19

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Rreb1 polyclonal antibody

  • Catalog # : PAB10322
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against recombinant Rreb1.
  • Immunogen:
  • Recombinant protein corresponding to mouse Rreb1.
  • Host:
  • Rabbit
  • Reactivity:
  • Mouse
  • Specificity:
  • This Protein A purified antibody is directed against the mouse RREB1 protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:2000-1:10000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using Rreb1 polyclonal antibody (Cat # PAB10322) shows detection ofa predominant band believed to be Rreb1 invarious cell lysates (1 - HEK293, 2 - RFP-Rreb transfected HEK293, 3 - M460 and 4 - T1165).
    All lysates were loaded at 20 ug per lane and separated by SDS-PAGE.
    After transfer to nitrocellulose, the membrane was probed with the primary antibody diluted to 1:1,000.
    The membrane was washed and reacted with IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX.
    IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers as indicated.
    Personal Communication, Shuling Zhang, CCR, NCI, Bethesda, MD.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • Q4ZE88;AAX83010
  • Gene Name:
  • Rreb1
  • Gene Alias:
  • 1110037N09Rik,AA414966,B930013M22Rik
  • Gene Description:
  • ras responsive element binding protein 1
  • Other Designations:
  • -
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