Rreb1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using Rreb1 polyclonal antibody (Cat # PAB10322) shows detection ofa predominant band believed to be Rreb1 invarious cell lysates (1 - HEK293, 2 - RFP-Rreb transfected HEK293, 3 - M460 and 4 - T1165).
All lysates were loaded at 20 ug per lane and separated by SDS-PAGE.
After transfer to nitrocellulose, the membrane was probed with the primary antibody diluted to 1:1,000.
The membrane was washed and reacted with IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX.
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers as indicated.
Personal Communication, Shuling Zhang, CCR, NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant Rreb1.
Immunogen
Recombinant protein corresponding to mouse Rreb1.
Host
Rabbit
Reactivity
Mouse
Specificity
This Protein A purified antibody is directed against the mouse RREB1 protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:2000-1:10000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using Rreb1 polyclonal antibody (Cat # PAB10322) shows detection ofa predominant band believed to be Rreb1 invarious cell lysates (1 - HEK293, 2 - RFP-Rreb transfected HEK293, 3 - M460 and 4 - T1165).
All lysates were loaded at 20 ug per lane and separated by SDS-PAGE.
After transfer to nitrocellulose, the membrane was probed with the primary antibody diluted to 1:1,000.
The membrane was washed and reacted with IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX.
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers as indicated.
Personal Communication, Shuling Zhang, CCR, NCI, Bethesda, MD.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Rreb1
Entrez GeneID
68750Protein Accession#
Q4ZE88;AAX83010Gene Name
Rreb1
Gene Alias
1110037N09Rik, AA414966, B930013M22Rik
Gene Description
ras responsive element binding protein 1
Gene Ontology
HyperlinkOther Designations
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Publication Reference
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p16 INK4a gene promoter variation and differential binding of a repressor, the ras-responsive zinc-finger transcription factor, RREB.
Zhang S, Qian X, Redman C, Bliskovski V, Ramsay ES, Lowy DR, Mock BA.
Oncogene 2003 Apr; 22(15):2285.
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Cdkn2a, the cyclin-dependent kinase inhibitor encoding p16INK4a and p19ARF, is a candidate for the plasmacytoma susceptibility locus, Pctr1.
Zhang S, Ramsay ES, Mock BA.
PNAS 1998 Mar; 95(5):2429.
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p16 INK4a gene promoter variation and differential binding of a repressor, the ras-responsive zinc-finger transcription factor, RREB.
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