SUMO3 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis is shown using SUMO3 polyclonal antibody (Cat # PAB10320) to detect GFP-SUMO fusion proteins (arrowheads).
Panel A. Increasing concentrations of human GFP-SUMO3 were run on a SDS-PAGE, transferred onto nitrocellulose, and blocked for 1 hour with 5% non-fat dry milk in TTBS, and probed overnight at 4°C with a 1 : 1000 dilution of anti-SUMO3 antibody in 5% non-fat dry milk in TTBS.
Detection occurred using a 1 : 1,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.
A chemiluminescence system was used for signal detection.
Panel B. Specificity of the antibody was confirmed by SDS-PAGE of 5 ug of various GFP-SUMO constructs followed by transfer onto nitrocellulose.
Lanes : 1, MW marker. 2, GFP-human SUMO3. 3, GFP-human SUMO1. 4, GFP-yeast SUMO. 5, GFP-Arabidopsis thaliana SUMO1. 6, GFP- Arabidopsis thaliana SUMO2. 7, GFP-tomato SUMO.
After blocking for 1 hour with 5% non-fat dry milk in TTBS,the blot was probed overnight at 4°C with SUMO3 polyclonal antibody diluted and detected as above.
Only the human GFP-SUMO3 band was visualized by chemiluminescence, and no crossreactivity with other SUMO family members was observed.
-
Specification
Product Description
Rabbit polyclonal antibody raised against a recombinant SUMO3.
Immunogen
Recombinant protein corresponding to human SUMO3.
Host
Rabbit
Reactivity
Human
Form
Lyophilized
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:4000-1:20000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot after reconstitution to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Recombinant protein)
Western blot analysis is shown using SUMO3 polyclonal antibody (Cat # PAB10320) to detect GFP-SUMO fusion proteins (arrowheads).
Panel A. Increasing concentrations of human GFP-SUMO3 were run on a SDS-PAGE, transferred onto nitrocellulose, and blocked for 1 hour with 5% non-fat dry milk in TTBS, and probed overnight at 4°C with a 1 : 1000 dilution of anti-SUMO3 antibody in 5% non-fat dry milk in TTBS.
Detection occurred using a 1 : 1,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.
A chemiluminescence system was used for signal detection.
Panel B. Specificity of the antibody was confirmed by SDS-PAGE of 5 ug of various GFP-SUMO constructs followed by transfer onto nitrocellulose.
Lanes : 1, MW marker. 2, GFP-human SUMO3. 3, GFP-human SUMO1. 4, GFP-yeast SUMO. 5, GFP-Arabidopsis thaliana SUMO1. 6, GFP- Arabidopsis thaliana SUMO2. 7, GFP-tomato SUMO.
After blocking for 1 hour with 5% non-fat dry milk in TTBS,the blot was probed overnight at 4°C with SUMO3 polyclonal antibody diluted and detected as above.
Only the human GFP-SUMO3 band was visualized by chemiluminescence, and no crossreactivity with other SUMO family members was observed.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
-
Gene Info — SUMO3
Entrez GeneID
6612Protein Accession#
NP_008867Gene Name
SUMO3
Gene Alias
SMT3A, SMT3H1, SUMO-3
Gene Description
SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae)
Omim ID
602231Gene Ontology
HyperlinkGene Summary
SUMO proteins, such as SUMO3, and ubiquitin (see MIM 191339) posttranslationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability (Su and Li, 2002 [PubMed 12383504]).[supplied by OMIM
Other Designations
OTTHUMP00000115275|SMT3 suppressor of mif two 3 homolog 1|small ubiquitin-like modifier protein 3
-
Interactome
-
Disease
-
Publication Reference
-
A synergy control motif within the attenuator domain of CCAAT/enhancer-binding protein alpha inhibits transcriptional synergy through its PIASy-enhanced modification by SUMO-1 or SUMO-3.
Subramanian L, Benson MD, Iñiguez-Lluhí JA.
The Journal of Biological Chemistry 2003 Mar; 278(11):9134.
-
Molecular features of human ubiquitin-like SUMO genes and their encoded proteins.
Su HL, Li SS.
Gene 2002 Aug; 296(1-2):65.
-
SUMO, ubiquitin's mysterious cousin.
Muller S, Hoege C, Pyrowolakis G, Jentsch S.
Nature Reviews. Molecular Cell Biology 2001 Mar; 2(3):202.
-
A synergy control motif within the attenuator domain of CCAAT/enhancer-binding protein alpha inhibits transcriptional synergy through its PIASy-enhanced modification by SUMO-1 or SUMO-3.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com