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Last updated: 2017/2/19
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SUMO3 polyclonal antibody

  • Catalog # : PAB10320
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against a recombinant SUMO3.
  • Immunogen:
  • Recombinant protein corresponding to human SUMO3.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Lyophilized
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:4000-1:20000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot after reconstitution to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis is shown using SUMO3 polyclonal antibody (Cat # PAB10320) to detect GFP-SUMO fusion proteins (arrowheads).
    Panel A. Increasing concentrations of human GFP-SUMO3 were run on a SDS-PAGE, transferred onto nitrocellulose, and blocked for 1 hour with 5% non-fat dry milk in TTBS, and probed overnight at 4°C with a 1 : 1000 dilution of anti-SUMO3 antibody in 5% non-fat dry milk in TTBS.
    Detection occurred using a 1 : 1,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.
    A chemiluminescence system was used for signal detection.
    Panel B. Specificity of the antibody was confirmed by SDS-PAGE of 5 ug of various GFP-SUMO constructs followed by transfer onto nitrocellulose.
    Lanes : 1, MW marker. 2, GFP-human SUMO3. 3, GFP-human SUMO1. 4, GFP-yeast SUMO. 5, GFP-Arabidopsis thaliana SUMO1. 6, GFP- Arabidopsis thaliana SUMO2. 7, GFP-tomato SUMO.
    After blocking for 1 hour with 5% non-fat dry milk in TTBS,the blot was probed over
  • Immunofluorescence
  • ELISA
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunofluorescence
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 6612
  • Gene Name:
  • SUMO3
  • Gene Alias:
  • SMT3A,SMT3H1,SUMO-3
  • Gene Description:
  • SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae)
  • Gene Summary:
  • SUMO proteins, such as SUMO3, and ubiquitin (see MIM 191339) posttranslationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability (Su and Li, 2002 [PubMed 12383504]).[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000115275,SMT3 suppressor of mif two 3 homolog 1,small ubiquitin-like modifier protein 3
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