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Last updated: 2016/10/23
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SUMO1 polyclonal antibody

  • Catalog # : PAB10319
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against a recombinant SUMO1.
  • Immunogen:
  • Recombinant protein corresponding to human SUMO1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:4000-1:20000)
    Western Blot (1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Immunoblot of SUMO1 polyclonal antibody (Cat # PAB10319), generated by immunizationwith recombinant human SUMO, was tested by immunoblot against a SUMO-GFP fusion proteinafter cleavage by proteases.
    Dilution of the antibody between 1 : 1,000 and 1 : 5,000 showed strong reactivity specifically with the SUMO portion of the fusion protein (arrowhead).
    In this blot the antibody was used at a 1 : 2000 dilution incubated overnight at 4°C in 5% non-fat dry milk in TTBS.
    Detection occurred using a 1 : 2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.
    Achemiluminescence system was used for signal detection.
    Data contributed by M.Malakhov,, personal communication.
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 7341
  • Gene Name:
  • SUMO1
  • Gene Alias:
  • Gene Description:
  • SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
  • Gene Summary:
  • This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq
  • Other Designations:
  • GAP modifying protein 1,SMT3 suppressor of mif two 3 homolog 1,sentrin,ubiquitin-like 1 (sentrin)
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