Immunoblot of SUMO1 polyclonal antibody (Cat # PAB10319), generated by immunizationwith recombinant human SUMO, was tested by immunoblot against a SUMO-GFP fusion proteinafter cleavage by proteases. Dilution of the antibody between 1 : 1,000 and 1 : 5,000 showed strong reactivity specifically with the SUMO portion of the fusion protein (arrowhead). In this blot the antibody was used at a 1 : 2000 dilution incubated overnight at 4°C in 5% non-fat dry milk in TTBS. Detection occurred using a 1 : 2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. Achemiluminescence system was used for signal detection. Data contributed by M.Malakhov, www.lifesensors.com, personal communication.
This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq
GAP modifying protein 1,SMT3 suppressor of mif two 3 homolog 1,sentrin,ubiquitin-like 1 (sentrin)