SUMO1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against a recombinant SUMO1.
Immunogen
Recombinant protein corresponding to human SUMO1.
Host
Rabbit
Reactivity
Human
Form
Lyophilized
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:4000-1:20000)
Western Blot (1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.02 M KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Immunoblot of SUMO1 polyclonal antibody (Cat # PAB10319), generated by immunizationwith recombinant human SUMO, was tested by immunoblot against a SUMO-GFP fusion proteinafter cleavage by proteases.
Dilution of the antibody between 1 : 1,000 and 1 : 5,000 showed strong reactivity specifically with the SUMO portion of the fusion protein (arrowhead).
In this blot the antibody was used at a 1 : 2000 dilution incubated overnight at 4°C in 5% non-fat dry milk in TTBS.
Detection occurred using a 1 : 2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.
Achemiluminescence system was used for signal detection.
Data contributed by M.Malakhov, www.lifesensors.com, personal communication.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SUMO1
Entrez GeneID
7341Gene Name
SUMO1
Gene Alias
DAP-1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, SUMO-1, UBL1
Gene Description
SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
Omim ID
601912Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq
Other Designations
GAP modifying protein 1|SMT3 suppressor of mif two 3 homolog 1|sentrin|ubiquitin-like 1 (sentrin)
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Interactome
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Disease
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Publication Reference
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SP-RING for SUMO: new functions bloom for a ubiquitin-like protein.
Hochstrasser M.
Cell 2001 Oct; 107(1):5.
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Involvement of PIAS1 in the sumoylation of tumor suppressor p53.
Kahyo T, Nishida T, Yasuda H.
Molecular Cell 2001 Sep; 8(3):713.
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SUMO, ubiquitin's mysterious cousin.
Muller S, Hoege C, Pyrowolakis G, Jentsch S.
Nature Reviews. Molecular Cell Biology 2001 Mar; 2(3):202.
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SP-RING for SUMO: new functions bloom for a ubiquitin-like protein.
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