Immunoblot of AHSG polyclonal antibody (Cat # PAB10313), generated by immunization with mature protein, was tested by immunoblot against AHSG in purified preparations and in human plasma. Lane 1 contains 250 ng of purified human AHSG. Lane 2 contains 5 uL of a 1 : 50 dilution of human serum. Dilution of Anti-human AHSG antibody between 1 : 10,000 and 1 : 20,000 showed strong reactivity by immunoblot. In this blot the antibody was used at a 1 : 10,000 dilution incubated 1 h at room temperature in 1% BSA in TTBS. Detection occurred using a 1 : 5,000 dilution of IRDye™800 conjugated Donkey anti-Goat IgG for 45 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.
Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure. [provided by RefSeq