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Last updated: 2017/3/26
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PDCD4 polyclonal antibody

  • Catalog # : PAB10308
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of PDCD4.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human PDCD4.
  • Host:
  • Rabbit
  • Reactivity:
  • Clawed frog, Human, Mouse, Rat
  • Specificity:
  • This antibody reacts with Pdcd4 protein that is either phosphorylated or non-phosphorylated at Ser457.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:10000-1:50000)
    Western Blot (1:1000-1:10000)
    Immunohistochemistry (1:100-1:300)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western blot using PDCD4 polyclonal antibody (Cat # PAB10308) shows detection of a band ~52 kDa in size corresponding to PDCD4 (arrowhead).
    Lane 1 contains recombinant PDCD4.
    Lane 2 contains 293 HEK cells treated with TPA and MG132.
    The PDCD4 polyclonal antibody was used at a 1 : 5,000 dilution.
    Personal Communication. M Young & A Jansen, NCI, Bethesda, MD.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • PDCD4 polyclonal antibody (Cat # PAB10308) was used at a 1 : 100 dilution to detect PDCD4 by immunohistochemistry on mouse colon tissue.
    Tissue was fixed in 4% paraformaldehyde and paraffin embedded.
    Tissue sections were deparaffinized and treated by trypsinization before staining.
    Personal Communication. M Young, NCI, Bethesda, MD.
  • ELISA
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_055271;O15501
  • Gene Name:
  • PDCD4
  • Gene Alias:
  • H731,MGC33046,MGC33047
  • Gene Description:
  • programmed cell death 4 (neoplastic transformation inhibitor)
  • Gene Summary:
  • This gene encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two transcripts encoding different isoforms have been identified. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000020483,nuclear antigen H731,programmed cell death 4
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