PDCD4 polyclonal antibody
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More Files
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Western Blot
Western blot using PDCD4 polyclonal antibody (Cat # PAB10308) shows detection of a band ~52 kDa in size corresponding to PDCD4 (arrowhead).
Lane 1 contains recombinant PDCD4.
Lane 2 contains 293 HEK cells treated with TPA and MG132.
The PDCD4 polyclonal antibody was used at a 1 : 5,000 dilution.
Personal Communication. M Young & A Jansen, NCI, Bethesda, MD.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
PDCD4 polyclonal antibody (Cat # PAB10308) was used at a 1 : 100 dilution to detect PDCD4 by immunohistochemistry on mouse colon tissue.
Tissue was fixed in 4% paraformaldehyde and paraffin embedded.
Tissue sections were deparaffinized and treated by trypsinization before staining.
Personal Communication. M Young, NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PDCD4.
Immunogen
A synthetic peptide corresponding to C-terminus of human PDCD4.
Host
Rabbit
Reactivity
Clawed frog, Human, Mouse, Rat
Specificity
This antibody reacts with Pdcd4 protein that is either phosphorylated or non-phosphorylated at Ser457.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:50000)
Western Blot (1:1000-1:10000)
Immunohistochemistry (1:100-1:300)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot using PDCD4 polyclonal antibody (Cat # PAB10308) shows detection of a band ~52 kDa in size corresponding to PDCD4 (arrowhead).
Lane 1 contains recombinant PDCD4.
Lane 2 contains 293 HEK cells treated with TPA and MG132.
The PDCD4 polyclonal antibody was used at a 1 : 5,000 dilution.
Personal Communication. M Young & A Jansen, NCI, Bethesda, MD.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
PDCD4 polyclonal antibody (Cat # PAB10308) was used at a 1 : 100 dilution to detect PDCD4 by immunohistochemistry on mouse colon tissue.
Tissue was fixed in 4% paraformaldehyde and paraffin embedded.
Tissue sections were deparaffinized and treated by trypsinization before staining.
Personal Communication. M Young, NCI, Bethesda, MD.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PDCD4
Entrez GeneID
27250Protein Accession#
NP_055271;O15501Gene Name
PDCD4
Gene Alias
H731, MGC33046, MGC33047
Gene Description
programmed cell death 4 (neoplastic transformation inhibitor)
Omim ID
608610Gene Ontology
HyperlinkGene Summary
This gene encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two transcripts encoding different isoforms have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000020483|nuclear antigen H731|programmed cell death 4
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Interactome
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Disease
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Publication Reference
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Clinical significance of decreased programmed cell death 4 expression in patients with giant cell tumors of the bone.
Gao F, Zhang W, Ding L, Zhao M, Ma Z, Huang S.
Oncology Letters 2018 Sep; 16(3):3805.
Application：IHC-P, Human, Giant cell tumor of the bone.
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Akt phosphorylates and regulates Pdcd4 tumor suppressor protein.
Palamarchuk A, Efanov A, Maximov V, Aqeilan RI, Croce CM, Pekarsky Y.
Cancer Research 2005 Dec; 65(24):11282.
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Epidermal expression of the translation inhibitor programmed cell death 4 suppresses tumorigenesis.
Jansen AP, Camalier CE, Colburn NH.
Cancer Research 2005 Jul; 65(14):6034.
Application：IHC-P, WB-Ce, WB-Tr, Mouse, Primary keratinocytes, Skin.
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Clinical significance of decreased programmed cell death 4 expression in patients with giant cell tumors of the bone.
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