Western blot using PDCD4 polyclonal antibody (Cat # PAB10308) shows detection of a band ~52 kDa in size corresponding to PDCD4 (arrowhead). Lane 1 contains recombinant PDCD4. Lane 2 contains 293 HEK cells treated with TPA and MG132. The PDCD4 polyclonal antibody was used at a 1 : 5,000 dilution. Personal Communication. M Young & A Jansen, NCI, Bethesda, MD.
PDCD4 polyclonal antibody (Cat # PAB10308) was used at a 1 : 100 dilution to detect PDCD4 by immunohistochemistry on mouse colon tissue. Tissue was fixed in 4% paraformaldehyde and paraffin embedded. Tissue sections were deparaffinized and treated by trypsinization before staining. Personal Communication. M Young, NCI, Bethesda, MD.
This gene encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two transcripts encoding different isoforms have been identified. [provided by RefSeq
OTTHUMP00000020483,nuclear antigen H731,programmed cell death 4