Product Browser

Last updated: 2017/6/18
  • Related Product Showcase

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

PACRG polyclonal antibody

  • Catalog # : PAB10304
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of PACRG.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 204-215 of human PACRG.
  • Host:
  • Rabbit
  • Reactivity:
  • Chicken, Human, Mouse, Rat
  • Specificity:
  • This affinity purified antibody is directed against human PACRG protein. Expect reactivity with both splice variants of this protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:2000-1:10000)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • 1.
  • Susceptibility to leprosy is associated with PARK2 and PACRG.
    Mira MT, Alcais A, Nguyen VT, Moraes MO, Di Flumeri C, Vu HT, Mai CP, Nguyen TH, Nguyen NB, Pham XK, Sarno EN, Alter A, Montpetit A, Moraes ME, Moraes JR, Dore C, Gallant CJ, Lepage P, Verner A, Van De Vosse E, Hudson TJ, Abel L, Schurr E.Nature. 2004 Feb 12;427(6975):636-40. Epub 2004 Jan 25.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using PACRG polyclonal antibody (Cat # PAB10304) shows detection of aband ~ 40 KDa corresponding to human PACRG (arrowhead lane 1). Specific reactivity with this bandis blocked when the antibody is pre-incubated withthe immunizing peptide (Lane 2).
    Approximately 35 ug of a mouse embryonic fibroblast (MEF) whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.
    After blocking the membrane was probed with the primary antibody diluted to 1:1,500 for 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
    IRDye™800 fluorescence image was captured usingthe Odyssey® Infrared Imaging System developed byLI-COR.
    IRDye is a trademark of LI-COR, Inc.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • Q96M98;AAH30642
  • Gene Name:
  • PACRG
  • Gene Alias:
  • FLJ32724,GLUP,HAK005771,PARK2CRG,RP3-495O10.2
  • Gene Description:
  • PARK2 co-regulated
  • Gene Summary:
  • This gene encodes a protein that is conserved across metazoans. In vertebrates, this gene is linked in a head-to-head arrangement with the adjacent parkin gene, which is associated with autosomal recessive juvenile Parkinson's disease. These genes are co-regulated in various tissues and they share a bi-directional promoter. Both genes are associated with susceptibility to leprosy. The parkin co-regulated gene protein forms a large molecular complex with chaperones, including heat shock proteins 70 and 90, and chaperonin components. This protein is also a component of Lewy bodies in Parkinson's disease patients, and it suppresses unfolded Pael receptor-induced neuronal cell death. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000017729,OTTHUMP00000017730,molecular chaperone/chaperonin-binding protein,parkin co-regulated gene protein
  • RSS
  • YouTube
  • Linkedin
  • Facebook