Nrf1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot using Nrf1 polyclonal antibody (Cat # PAB10295) shows detection of a 67 KDa band corresponding to human Nrf1 in a (Lane 1) HeLa nuclear extract and (Lane 2) whole cell lysate (molecular weight marker not shown).
Approximately 10 ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose.
The blot was incubated with a 1 : 500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye™700 labeled Goat-a-Rabbit IgG [H&L] diluted 1 : 2,500.
IRDye™700 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is atrademark of LI-COR, Inc.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of Nrf1 polyclonal antibody (Cat # PAB10295) was used at a 5 ug/mL to detect NRF1 in a variety of tissues.
This image shows Nrf1 staining of human testis.
The antibody shows nuclear staining in lymphocytes.
Tissue was formalin-fixed andparaffin embedded.
Personal Communication, Tina Roush,Life Span Biosciences, Seattle, WA.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant Nrf1.
Immunogen
Recombinant protein corresponding to amino acids 1-534 of mouse Nrf1.
Host
Rabbit
Reactivity
Chicken, Chimpanzee, Dog, Frog, Human, Mouse, Rat
Specificity
This protein A purified antibody is directed against mouse NRF1.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:3000-1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using Nrf1 polyclonal antibody (Cat # PAB10295) shows detection of a 67 KDa band corresponding to human Nrf1 in a (Lane 1) HeLa nuclear extract and (Lane 2) whole cell lysate (molecular weight marker not shown).
Approximately 10 ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose.
The blot was incubated with a 1 : 500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye™700 labeled Goat-a-Rabbit IgG [H&L] diluted 1 : 2,500.
IRDye™700 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is atrademark of LI-COR, Inc.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of Nrf1 polyclonal antibody (Cat # PAB10295) was used at a 5 ug/mL to detect NRF1 in a variety of tissues.
This image shows Nrf1 staining of human testis.
The antibody shows nuclear staining in lymphocytes.
Tissue was formalin-fixed andparaffin embedded.
Personal Communication, Tina Roush,Life Span Biosciences, Seattle, WA.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Nrf1
Entrez GeneID
18181Protein Accession#
Q9WU00;AAH05410Gene Name
Nrf1
Gene Alias
C87038, D6Ertd415e
Gene Description
nuclear respiratory factor 1
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000015186|OTTMUSP00000015187|OTTMUSP00000015190
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Publication Reference
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Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M.
Cell 2003 Nov; 115(5):629.
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Mitochondrial benzodiazepine receptors regulate oxygen homeostasis in the early mouse embryo.
O'Hara MF, Nibbio BJ, Craig RC, Nemeth KR, Charlap JH, Knudsen TB.
Reproductive Toxicology 2003 Jul; 17(4):365.
Application：Func, Mouse, Mouse embryos.
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c-MYC apoptotic function is mediated by NRF-1 target genes.
Morrish F, Giedt C, Hockenbery D.
Genes & Development 2003 Jan; 17(2):240.
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Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
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