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Last updated: 2017/11/19

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Nrf1 polyclonal antibody

  • Catalog # : PAB10295
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial recombinant Nrf1.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1-534 of mouse Nrf1.
  • Host:
  • Rabbit
  • Reactivity:
  • Chicken, Chimpanzee, Dog, Frog, Human, Mouse, Rat
  • Specificity:
  • This protein A purified antibody is directed against mouse NRF1.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:3000-1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (2-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using Nrf1 polyclonal antibody (Cat # PAB10295) shows detection of a 67 KDa band corresponding to human Nrf1 in a (Lane 1) HeLa nuclear extract and (Lane 2) whole cell lysate (molecular weight marker not shown).
    Approximately 10 ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose.
    The blot was incubated with a 1 : 500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye™700 labeled Goat-a-Rabbit IgG [H&L] diluted 1 : 2,500.
    IRDye™700 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDye is atrademark of LI-COR, Inc.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of Nrf1 polyclonal antibody (Cat # PAB10295) was used at a 5 ug/mL to detect NRF1 in a variety of tissues.
    This image shows Nrf1 staining of human testis.
    The antibody shows nuclear staining in lymphocytes.
    Tissue was formalin-fixed andparaffin embedded.
    Personal Communication, Tina Roush,Life Span Biosciences, Seattle, WA.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • Q9WU00;AAH05410
  • Gene Name:
  • Nrf1
  • Gene Alias:
  • C87038,D6Ertd415e
  • Gene Description:
  • nuclear respiratory factor 1
  • Other Designations:
  • OTTMUSP00000015186,OTTMUSP00000015187,OTTMUSP00000015190
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