Western blot using Nrf1 polyclonal antibody (Cat # PAB10295) shows detection of a 67 KDa band corresponding to human Nrf1 in a (Lane 1) HeLa nuclear extract and (Lane 2) whole cell lysate (molecular weight marker not shown). Approximately 10 ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1 : 500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye™700 labeled Goat-a-Rabbit IgG [H&L] diluted 1 : 2,500. IRDye™700 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is atrademark of LI-COR, Inc.
Immunohistochemistry of Nrf1 polyclonal antibody (Cat # PAB10295) was used at a 5 ug/mL to detect NRF1 in a variety of tissues. This image shows Nrf1 staining of human testis. The antibody shows nuclear staining in lymphocytes. Tissue was formalin-fixed andparaffin embedded. Personal Communication, Tina Roush,Life Span Biosciences, Seattle, WA.