Rabbit polyclonal antibody raised against synthetic peptide of NOTCH2.
A synthetic peptide corresponding to amino acids 2396-2409 of human NOTCH2.
Chimpanzee, Dog, Human
This antiserum is directed against human NOTCH 2. Only one (1) amino acid difference is found in mouse and this change is non-conservative. Based on the sequence we expect this antibody to react as well with rat and mouse NOTCH 2.
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
ELISA (1:30000-1:90000) Western Blot (1:400-1:2000) The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.09% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot using NOTCH2 polyclonal antibody (Cat # PAB10294) (intra) antibody shows detection of a band at ~110 KDa correspondingto active NOTCH2 protein (arrowhead). Western Blot analysis was performed for NOTCH2 expression using 100 ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active NOTCH2 (intra cellular NOTCH2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar timepoints. The band at about 110 kD represents active NOTCH2. This band is not seen in the control cell. The intracellular domain of NOTCH2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milkand probed with NOTCH2 polyclonal antibody at a 1 : 400 dilution.
Immunohistochemical staining with NOTCH2 polyclonal antibody (Cat # PAB10294) was diluted 1 : 500 to detect NOTCH 2 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. [provided by RefSeq