NOTCH2 polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining with NOTCH2 polyclonal antibody (Cat # PAB10293) was diluted 1 : 500 to detect NOTCH 2 in human liver tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NOTCH2.
Immunogen
A synthetic peptide corresponding to N-terminus the cleaved N intracellular domain (NICD) of human NOTCH2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antiserum is directed against human NOTCH 2. Based on sequences, we expect this antibody to react as well with rat and mouse NOTCH 2. Only one amino acid difference (conservative) occurs in mouse and rat. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Sandwich ELISA (1:30000-1:90000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining with NOTCH2 polyclonal antibody (Cat # PAB10293) was diluted 1 : 500 to detect NOTCH 2 in human liver tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NOTCH2
Entrez GeneID
4853Protein Accession#
Q04721(human);O35516(mouse);Q9QW30(rat);OMIMref:600275Gene Name
NOTCH2
Gene Alias
AGS2, hN2
Gene Description
Notch homolog 2 (Drosophila)
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. [provided by RefSeq
Other Designations
OTTHUMP00000014035|OTTHUMP00000059536|notch 2
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Interactome
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Pathway
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Disease
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Publication Reference
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Notch signaling and inherited disease syndromes.
Gridley T.
Human Molecular Genetics 2003 Apr; 12 Spec No 1:R9.
Application：Flow Cyt, WB, Human, Cancers, Mammalian cells.
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Notch-1 and Notch-2 exhibit unique patterns of expression in human B-lineage cells.
Bertrand FE, Eckfeldt CE, Lysholm AS, LeBien TW.
Leukemia 2000 Dec; 14(12):2095.
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Notch signaling and inherited disease syndromes.
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