Immunocytochemistry of RELA polyclonal antibody (Cat # PAB10290) was used at a 1 : 200 dilution to detect RELA in (Lane 1) control DU 145 cells and (Lane 2) TNF stimulated DU 145 cells. Although DU 145 show relatively high basal levels of nuclear RELA staining, significant enhancement of nuclear staining is seen in pB as evidence of translocation and availability of the NLS to be bound by the antibody. Cultured cells shown above were formalin-fixed.
Immunofluorescence microscopy of RELA polyclonal antibody (Cat # PAB10290) was used at a 1 : 200 dilution to detect RELA in TNF stimulated DU 145 cells. Image shown is at a 1 : 400 magnification. Tissue was fixed and prepared as above.
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM
nuclear factor of kappa light polypeptide gene enhancer in B-cells 3,v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65)),v-rel reticuloendotheliosis viral oncogene homolog