RELA polyclonal antibody
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Immunocytochemistry
Immunocytochemistry of RELA polyclonal antibody (Cat # PAB10290) was used at a 1 : 200 dilution to detect RELA in (Lane 1) control DU 145 cells and (Lane 2) TNF stimulated DU 145 cells.
Although DU 145 show relatively high basal levels of nuclear RELA staining, significant enhancement of nuclear staining is seen in pB as evidence of translocation and availability of the NLS to be bound by the antibody.
Cultured cells shown above were formalin-fixed.
Immunofluorescence
Immunofluorescence microscopy of RELA polyclonal antibody (Cat # PAB10290) was used at a 1 : 200 dilution to detect RELA in TNF stimulated DU 145 cells. Image shown is at a 1 : 400 magnification.
Tissue was fixed and prepared as above.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RELA.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus end of human RELA.
Host
Rabbit
Reactivity
Human
Specificity
The epitope recognized overlaps the NLS of the p65 subunit of the NFkB heterodimer. Therefore, This antibody selectively binds to the activated form of NFkB.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:2000)
Gel Supershift Assay (0.5-1.0 ul per assay)
Immunohistochemistry (1:200)
Immunofluorescence (1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Immunocytochemistry
Immunocytochemistry of RELA polyclonal antibody (Cat # PAB10290) was used at a 1 : 200 dilution to detect RELA in (Lane 1) control DU 145 cells and (Lane 2) TNF stimulated DU 145 cells.
Although DU 145 show relatively high basal levels of nuclear RELA staining, significant enhancement of nuclear staining is seen in pB as evidence of translocation and availability of the NLS to be bound by the antibody.
Cultured cells shown above were formalin-fixed.Immunofluorescence
Immunofluorescence microscopy of RELA polyclonal antibody (Cat # PAB10290) was used at a 1 : 200 dilution to detect RELA in TNF stimulated DU 145 cells. Image shown is at a 1 : 400 magnification.
Tissue was fixed and prepared as above.Enzyme-linked Immunoabsorbent Assay
Gel Supershift Assay
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Gene Info — RELA
Entrez GeneID
5970Gene Name
RELA
Gene Alias
MGC131774, NFKB3, p65
Gene Description
v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Omim ID
164014Gene Ontology
HyperlinkGene Summary
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM
Other Designations
nuclear factor of kappa light polypeptide gene enhancer in B-cells 3|v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65))|v-rel reticuloendotheliosis viral oncogene homolog
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Interactome
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Pathway
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Disease
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Publication Reference
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Molecular mechanisms of NF-kappaB activation induced by bacterial lipopolysaccharide through Toll-like receptors.
Zhang G, Ghosh S.
Journal of Endotoxin Research 2000 Dec; 6(6):453.
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Hormonal regulation of the NF-kappaB signaling pathway.
Delfino F, Walker WH.
Molecular and Cellular Endocrinology 1999 Nov; 157(1-2):1.
Application：IF, WB-Ce, WB-Tr, Human, Mammalian cells.
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Dexamethasone attenuates NF-kappa B DNA binding activity without inducing I kappa B levels in rat brain in vivo.
Unlap MT, Jope RS.
Brain Research. Molecular Brain Research 1997 Apr; 45(1):83.
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Nuclear uptake control of NF-kappa B by MAD-3, an I kappa B protein present in the nucleus.
Zabel U, Henkel T, Silva MS, Baeuerle PA.
The EMBO Journal 1993 Jan; 12(1):201.
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Molecular mechanisms of NF-kappaB activation induced by bacterial lipopolysaccharide through Toll-like receptors.
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