Product Browser

Last updated: 2016/10/23
  • Related Product Showcase

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

RELA polyclonal antibody

  • Catalog # : PAB10288
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of RELA.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human RELA.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (1:2000-1:5000)
    Gel Supershift Assay (0.5-1.0 ul per assay)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In antiserum (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Immunoblot of HeLa cell extract.
    A predominant band ~65 kDa (arrowhead) corresponding to RELA is observed.
    All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature.
    The membrane was blocked in 5% dry milk for 2 h.
    After washing, a 1:5,000 dilution of RELA polyclonal antibody (Cat # PAB10288) was added to the membrane and incubated for 2 h.
    Washes with buffer were performed 4 times for 5' each.
    The immunoblot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1 : 2,000 for 1 h.
    Washes with TBS preceded color development.
  • Gel Supershift Assay
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gel Supershift Assay
  • Gene Information
  • Entrez GeneID:
  • 5970
  • Gene Name:
  • RELA
  • Gene Alias:
  • MGC131774,NFKB3,p65
  • Gene Description:
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • Gene Summary:
  • NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM
  • Other Designations:
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells 3,v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65)),v-rel reticuloendotheliosis viral oncogene homolog
  • RSS
  • YouTube
  • Linkedin
  • Facebook