RELA polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RELA.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human RELA.
Host
Rabbit
Reactivity
Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:2000-1:5000)
Gel Supershift Assay (0.5-1.0 ul per assay)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Immunoblot of HeLa cell extract.
A predominant band ~65 kDa (arrowhead) corresponding to RELA is observed.
All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature.
The membrane was blocked in 5% dry milk for 2 h.
After washing, a 1:5,000 dilution of RELA polyclonal antibody (Cat # PAB10288) was added to the membrane and incubated for 2 h.
Washes with buffer were performed 4 times for 5' each.
The immunoblot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1 : 2,000 for 1 h.
Washes with TBS preceded color development.Enzyme-linked Immunoabsorbent Assay
Gel Supershift Assay
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Gene Info — RELA
Entrez GeneID
5970Gene Name
RELA
Gene Alias
MGC131774, NFKB3, p65
Gene Description
v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Omim ID
164014Gene Ontology
HyperlinkGene Summary
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM
Other Designations
nuclear factor of kappa light polypeptide gene enhancer in B-cells 3|v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65))|v-rel reticuloendotheliosis viral oncogene homolog
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Interactome
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Pathway
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Disease
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Publication Reference
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Upregulation of HMG1 leads to melanoma inhibitory activity expression in malignant melanoma cells and contributes to their malignancy phenotype.
Poser I, Golob M, Buettner R, Bosserhoff AK.
Molecular and Cellular Biology 2003 Apr; 23(8):2991.
Application:GSA, IP, Human, Mel Im cells.
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Curcumin blocks cytokine-mediated NF-kappa B activation and proinflammatory gene expression by inhibiting inhibitory factor I-kappa B kinase activity.
Jobin C, Bradham CA, Russo MP, Juma B, Narula AS, Brenner DA, Sartor RB.
Journal of Immunology 1999 Sep; 163(6):3474.
Application:IF, Func, Human, Caco-2, HT-29 cells.
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Dexamethasone attenuates NF-kappa B DNA binding activity without inducing I kappa B levels in rat brain in vivo.
Unlap MT, Jope RS.
Brain Research. Molecular Brain Research 1997 Apr; 45(1):83.
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Upregulation of HMG1 leads to melanoma inhibitory activity expression in malignant melanoma cells and contributes to their malignancy phenotype.
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