NFKB1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NFKB1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus human NFKB1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
Gel Supershift Assay (0.5-1.0 ul per assay)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Immunoblot of HeLa cell extract.
All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature.
The membrane was blocked in 5% dry milk for 2 h.
After washing, a 1 : 1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h.
Washes with buffer were performed 4 times for 5' each.
The immunoblot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1 : 2,000 for 1 h.
Washes with TBS preceded color development.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NFKB1
Entrez GeneID
4790Gene Name
NFKB1
Gene Alias
DKFZp686C01211, EBP-1, KBF1, MGC54151, NF-kappa-B, NFKB-p105, NFKB-p50, p105, p50
Gene Description
nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
Omim ID
164011Gene Ontology
HyperlinkGene Summary
This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
DNA binding factor KBF1|NF-kappabeta|nuclear factor NF-kappa-B p50 subunit|nuclear factor kappa-B DNA binding subunit|nuclear factor kappa-B, subunit 1
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Interactome
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Pathway
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Disease
- Abortion
- Acute Lung Injury
- Adenocarcinoma
- Alcoholism
- Alzheimer disease
- Arthritis
- Asthma
- Atherosclerosis
- Behcet Syndrome
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