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Last updated: 2017/7/23
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NEDD4 polyclonal antibody

  • Catalog # : PAB10283
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of NEDD4.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human NEDD4.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Chimpanzee, Dog, Horse, Human, Macaque, Rabbit
  • Specificity:
  • This antibody reacts with endogenous Nedd4 protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:20000-1:35000)
    Western Blot (1:300-1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using NEDD4 polyclonal antibody (Cat # PAB10283) shows detection of a 115 kDa band corresponding to endogenous NEDD4 (arrowhead) in MDA-MB-435 cell lysates.
    The blot was blocked with 5% BLOTTO overnight at 4°C.
    Primary antibody was used at a 1 : 350 dilution in 5% BLOTTO followed by reaction with a 1 : 20,000 dilution of HRP goat anti-rabbit IgG.
    ECL was used for detection.
  • Immunofluorescence
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunofluorescence
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4734
  • Protein Accession#:
  • NP_006145;P46934
  • Gene Name:
  • NEDD4
  • Gene Alias:
  • KIAA0093,MGC176705,NEDD4-1,RPF1
  • Gene Description:
  • neural precursor cell expressed, developmentally down-regulated 4
  • Other Designations:
  • receptor-potentiating factor 1
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