NEDD4 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using NEDD4 polyclonal antibody (Cat # PAB10283) shows detection of a 115 kDa band corresponding to endogenous NEDD4 (arrowhead) in MDA-MB-435 cell lysates.
The blot was blocked with 5% BLOTTO overnight at 4°C.
Primary antibody was used at a 1 : 350 dilution in 5% BLOTTO followed by reaction with a 1 : 20,000 dilution of HRP goat anti-rabbit IgG.
ECL was used for detection.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NEDD4.
Immunogen
A synthetic peptide corresponding to internal region of human NEDD4.
Host
Rabbit
Reactivity
Bovine, Chimpanzee, Dog, Horse, Human, Macaque, Rabbit
Specificity
This antibody reacts with endogenous Nedd4 protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:20000-1:35000)
Western Blot (1:300-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using NEDD4 polyclonal antibody (Cat # PAB10283) shows detection of a 115 kDa band corresponding to endogenous NEDD4 (arrowhead) in MDA-MB-435 cell lysates.
The blot was blocked with 5% BLOTTO overnight at 4°C.
Primary antibody was used at a 1 : 350 dilution in 5% BLOTTO followed by reaction with a 1 : 20,000 dilution of HRP goat anti-rabbit IgG.
ECL was used for detection.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — NEDD4
Entrez GeneID
4734Protein Accession#
NP_006145;P46934Gene Name
NEDD4
Gene Alias
KIAA0093, MGC176705, NEDD4-1, RPF1
Gene Description
neural precursor cell expressed, developmentally down-regulated 4
Omim ID
602278Gene Ontology
HyperlinkGene Summary
O
Other Designations
receptor-potentiating factor 1
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Interactome
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Pathway
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Disease
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Publication Reference
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NEDD4-2 (neural precursor cell expressed, developmentally down-regulated 4-2) negatively regulates TGF-beta (transforming growth factor-beta) signalling by inducing ubiquitin-mediated degradation of Smad2 and TGF-beta type I receptor.
Kuratomi G, Komuro A, Goto K, Shinozaki M, Miyazawa K, Miyazono K, Imamura T.
The Biochemical Journal 2005 Mar; 386(Pt 3):461.
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Nedd4-like proteins: an emerging family of ubiquitin-protein ligases implicated in diverse cellular functions.
Harvey KF, Kumar S.
Trends in Cell Biology 1999 May; 9(5):166.
Application：IHC, WB-Ce, WB-Tr, Human, Mammalian cells.
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Nedd4 mediates control of an epithelial Na+ channel in salivary duct cells by cytosolic Na+.
Dinudom A, Harvey KF, Komwatana P, Young JA, Kumar S, Cook DI.
PNAS 1998 Jun; 95(12):7169.
Application：IHC-P, Mouse, Mandibular gland.
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NEDD4-2 (neural precursor cell expressed, developmentally down-regulated 4-2) negatively regulates TGF-beta (transforming growth factor-beta) signalling by inducing ubiquitin-mediated degradation of Smad2 and TGF-beta type I receptor.
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