MYO1G polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MYO1G.
Immunogen
A synthetic peptide corresponding to internal region of human MYO1G.
Host
Rabbit
Reactivity
Chimpanzee, Human, Mouse, Rat
Specificity
This affinity-purified antibody is directed against human Myosin 1G protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:40000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using MYO1G polyclonal antibody (Cat # PAB10281) shows detection of a band ~100 kDa in size corresponding to MYO1G (arrowhead) in MYO1G positive whole cell lysate.
Lane 1 Jurkat, lane 2 peripheral blood T cells, lane 3 human spleen and lane 4 300.19.
Lane 5, 293 cells, appear negative for MYO1G.
Personal Communication. Stephen Shaw, NCI, Bethesda, MD.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — MYO1G
Entrez GeneID
64005Protein Accession#
NP_149043;Q96BE2(fragment)Gene Name
MYO1G
Gene Alias
HA-2, MGC142104
Gene Description
myosin IG
Gene Ontology
HyperlinkOther Designations
MYO1G variant protein|minor histocompatibility antigen HA-2
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Interactome
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Disease
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Publication Reference
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Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes.
Nebl T, Pestonjamasp KN, Leszyk JD, Crowley JL, Oh SW, Luna EJ.
The Journal of Biological Chemistry 2002 Nov; 277(45):43399.
Application:WB-Ce, Bovine, Neutrophils.
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Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes.
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