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Last updated: 2017/12/10
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MRE11 polyclonal antibody

  • Catalog # : PAB10277
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of MRE11.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 578-590 of Saccharomyces cerevisiae MRE11.
  • Host:
  • Rabbit
  • Reactivity:
  • Yeast
  • Specificity:
  • This affinity-purified antibody is directed against Saccharomyces cerevisiae Mre11 protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:10000-1:50000)
    Western Blot (1:200-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • ChIP
  • ChIP
  • Chromatin Immunoprecipitation (ChIP) using MRE11 polyclonal antibody (Cat # PAB10277).
    A yeast strain containing the HO endonuclease gene controlled by a galactose-inducible promoter (uninduced 0 m lanes) was shifted into galactose containing medium (induced 60 m lanes).
    After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of sheared chromatin.
    Chromatin was immunoprecipitated with the antibody at the stated dilutions.
    Immunocomplexes were captured using polyacrylamide bead linked secondary antibodies.
    The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower arrow) and 67 kb from the break (upper band, control locus).
    Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Protein Accession#:
  • P32829;NP_013951
  • Gene Name:
  • MRE11
  • Gene Alias:
  • NGS1,RAD58,XRS4
  • Gene Description:
  • Mre11p
  • Other Designations:
  • Subunit of a complex with Rad50p and Xrs2p (RMX complex) that functions in repair of DNA double-strand breaks and in telomere stability, exhibits nuclease activity that appears to be required for RMX function; widely conserved
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