Western blot using Mre11a polyclonal antibody (Cat # PAB10276) shows detection of a band ~ 80 KDa corresponding to mouse Mre11a (arrowhead). Lanes 1-4 contain 0.5 ug, 0.3 ug, 0.1 ug and 0.05 ug of purified mouse Mre11a protein, respectively. After 4-20% SDS-PAGE and transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:1,000 overnight at 4°C. The membrane was then washed and reacted with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.