Mre11a polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot using Mre11a polyclonal antibody (Cat # PAB10276) shows detection of a band ~ 80 KDa corresponding to mouse Mre11a (arrowhead).
Lanes 1-4 contain 0.5 ug, 0.3 ug, 0.1 ug and 0.05 ug of purified mouse Mre11a protein, respectively.
After 4-20% SDS-PAGE and transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:1,000 overnight at 4°C.
The membrane was then washed and reacted with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant Mre11a.
Immunogen
Recombinant protein corresponding to amino acids 68-706 of mouse Mre11a.
Host
Rabbit
Reactivity
Dog, Human, Macaque, Mouse, Rat
Specificity
This affinity purified antibody is directed against mouse Mre11 protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:10000-1:50000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot using Mre11a polyclonal antibody (Cat # PAB10276) shows detection of a band ~ 80 KDa corresponding to mouse Mre11a (arrowhead).
Lanes 1-4 contain 0.5 ug, 0.3 ug, 0.1 ug and 0.05 ug of purified mouse Mre11a protein, respectively.
After 4-20% SDS-PAGE and transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:1,000 overnight at 4°C.
The membrane was then washed and reacted with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Mre11a
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Publication Reference
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Disruption of mechanisms that prevent rereplication triggers a DNA damage response.
Archambault V, Ikui AE, Drapkin BJ, Cross FR.
Molecular and Cellular Biology 2005 Aug; 25(15):6707.
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Molecular basis of ataxia telangiectasia and related diseases.
Ball LG, Xiao W.
Acta Pharmacologica Sinica 2005 Aug; 26(8):897.
Application：WB, Human, Human mammalian cells.
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The Mre11/Rad50/Xrs2 complex and non-homologous end-joining of incompatible ends in S. cerevisiae.
Zhang X, Paull TT.
DNA Repair (Amst) 2005 Nov; 4(11):1281.
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Disruption of mechanisms that prevent rereplication triggers a DNA damage response.
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