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Mre11a polyclonal antibody

  • Catalog # : PAB10276
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial recombinant Mre11a.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 68-706 of mouse Mre11a.
  • Host:
  • Rabbit
  • Reactivity:
  • Dog, Human, Macaque, Mouse, Rat
  • Specificity:
  • This affinity purified antibody is directed against mouse Mre11 protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:10000-1:50000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot using Mre11a polyclonal antibody (Cat # PAB10276) shows detection of a band ~ 80 KDa corresponding to mouse Mre11a (arrowhead).
    Lanes 1-4 contain 0.5 ug, 0.3 ug, 0.1 ug and 0.05 ug of purified mouse Mre11a protein, respectively.
    After 4-20% SDS-PAGE and transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:1,000 overnight at 4°C.
    The membrane was then washed and reacted with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
    IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDye is a trademark of LI-COR, Inc.
  • ELISA
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • Mre11a
  • Gene Alias:
  • Mre11,Mre11b
  • Gene Description:
  • meiotic recombination 11 homolog A (S. cerevisiae)
  • Other Designations:
  • meiotic recombination 11 homolog A
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