Rabbit polyclonal antibody raised against synthetic peptide of Rif1.
A synthetic peptide corresponding to amino acids 2406-2419 of mouse Rif1.
Theoretical MW (kDa):
58.1, 265.7, 267.1
Chimpanzee, Dog, Human, Mouse, Rat
This affinity purified antibody is directed against mouse Rif1 protein. Databases confirm that the immunogen sequence represents the C-terminal end of mouse Rif1 (534 aa 58.1 KDa), mouse telomere-associated protein RIF1 (2418 aa 265.7 KDa) and mouse Rap1-interacting factor 1 (2426 267.1 KDa).
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
ELISA (1:5000-1:40000) Western Blot (1:500-1:3000) The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot using Rif1 polyclonal antibody (Cat # PAB10275) shows detection of a band ~ 265 KDa corresponding to mouse Rif1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 25 ug of MEF whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/mL for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.