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Rif1 polyclonal antibody

  • Catalog # : PAB10275
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Rif1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 2406-2419 of mouse Rif1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 58.1, 265.7, 267.1
  • Reactivity:
  • Chimpanzee, Dog, Human, Mouse, Rat
  • Specificity:
  • This affinity purified antibody is directed against mouse Rif1 protein. Databases confirm that the immunogen sequence represents the C-terminal end of mouse Rif1 (534 aa 58.1 KDa), mouse telomere-associated protein RIF1 (2418 aa 265.7 KDa) and mouse Rap1-interacting factor 1 (2426 267.1 KDa).
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:5000-1:40000)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using Rif1 polyclonal antibody (Cat # PAB10275) shows detection of a band ~ 265 KDa corresponding to mouse Rif1 (arrowhead).
    Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown).
    Approximately 25 ug of MEF whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose.
    After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/mL for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDye is a trademark of LI-COR, Inc.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • Q7TPD8;Q6PR54
  • Gene Name:
  • Rif1
  • Gene Alias:
  • 5730435J01Rik,6530403D07Rik,AU016181,AW549474,D2Ertd145e
  • Gene Description:
  • Rap1 interacting factor 1 homolog (yeast)
  • Other Designations:
  • telomere-associated protein RIF1
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