Rif1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using Rif1 polyclonal antibody (Cat # PAB10275) shows detection of a band ~ 265 KDa corresponding to mouse Rif1 (arrowhead).
Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown).
Approximately 25 ug of MEF whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/mL for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Rif1.
Immunogen
A synthetic peptide corresponding to amino acids 2406-2419 of mouse Rif1.
Host
Rabbit
Theoretical MW (kDa)
58.1, 265.7, 267.1
Reactivity
Chimpanzee, Dog, Human, Mouse, Rat
Specificity
This affinity purified antibody is directed against mouse Rif1 protein. Databases confirm that the immunogen sequence represents the C-terminal end of mouse Rif1 (534 aa 58.1 KDa), mouse telomere-associated protein RIF1 (2418 aa 265.7 KDa) and mouse Rap1-interacting factor 1 (2426 267.1 KDa).
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:5000-1:40000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using Rif1 polyclonal antibody (Cat # PAB10275) shows detection of a band ~ 265 KDa corresponding to mouse Rif1 (arrowhead).
Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown).
Approximately 25 ug of MEF whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/mL for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Rif1
Entrez GeneID
51869Protein Accession#
Q7TPD8;Q6PR54Gene Name
Rif1
Gene Alias
5730435J01Rik, 6530403D07Rik, AU016181, AW549474, D2Ertd145e
Gene Description
Rap1 interacting factor 1 homolog (yeast)
Gene Ontology
HyperlinkOther Designations
telomere-associated protein RIF1
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Publication Reference
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Identification and characterisation of mRif1: a mouse telomere-associated protein highly expressed in germ cells and embryo-derived pluripotent stem cells.
Adams IR, McLaren A.
Developmental Dynamics 2004 Apr; 229(4):733.
Application：ChIP, IF, IHC-Fr, WB, Mouse, Mouse cell lines, Embryos.
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Identification and characterisation of mRif1: a mouse telomere-associated protein highly expressed in germ cells and embryo-derived pluripotent stem cells.
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