Product Browser

Last updated: 2017/7/16
  • Related Product Showcase
  • By Research Area

  • By Disease

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

Mdm2 polyclonal antibody

  • Catalog # : PAB10274
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Mdm2.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 177-195 of mouse Mdm2.
  • Host:
  • Rabbit
  • Reactivity:
  • Mouse
  • Specificity:
  • Reactivity occurs against Mouse MDM2 protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:3000-1:12000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using Mdm2 polyclonal antibody (Cat # PAB10274) is shown to detect a band (arrow) corresponding to mouse Mdm2 protein present in mouse MEF cells (Lane 2), but not human kidney HEK293 cells (lane1).
    Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE.
    After blocking the membrane with 5% normal goat serum, 0.5% BLOTTO in PBS, the membrane was probed for overnight at 4° with the primary antibody diluted to 1:500 in 1% normal goat serum, 0.1% BLOTTO in PBS.
    The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at roomtemperature (800 nm channel, green).
    Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (700 nm channel, red).
    IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDye is a trademark of LI-COR, Inc.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Gene Name:
  • Mdm2
  • Gene Alias:
  • 1700007J15Rik,AA415488,Mdm-2
  • Gene Description:
  • transformed mouse 3T3 cell double minute 2
  • Other Designations:
  • OTTMUSP00000022916
  • RSS
  • YouTube
  • Linkedin
  • Facebook