Mdm2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot using Mdm2 polyclonal antibody (Cat # PAB10274) is shown to detect a band (arrow) corresponding to mouse Mdm2 protein present in mouse MEF cells (Lane 2), but not human kidney HEK293 cells (lane1).
Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE.
After blocking the membrane with 5% normal goat serum, 0.5% BLOTTO in PBS, the membrane was probed for overnight at 4° with the primary antibody diluted to 1:500 in 1% normal goat serum, 0.1% BLOTTO in PBS.
The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at roomtemperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (700 nm channel, red).
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Mdm2.
Immunogen
A synthetic peptide corresponding to amino acids 177-195 of mouse Mdm2.
Host
Rabbit
Reactivity
Mouse
Specificity
Reactivity occurs against Mouse MDM2 protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:3000-1:12000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using Mdm2 polyclonal antibody (Cat # PAB10274) is shown to detect a band (arrow) corresponding to mouse Mdm2 protein present in mouse MEF cells (Lane 2), but not human kidney HEK293 cells (lane1).
Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE.
After blocking the membrane with 5% normal goat serum, 0.5% BLOTTO in PBS, the membrane was probed for overnight at 4° with the primary antibody diluted to 1:500 in 1% normal goat serum, 0.1% BLOTTO in PBS.
The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at roomtemperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (700 nm channel, red).
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — Mdm2
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Publication Reference
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Repression of the Arf tumor suppressor by E2F3 is required for normal cell cycle kinetics.
Aslanian A, Iaquinta PJ, Verona R, Lees JA.
Genes & Development 2004 Jun; 18(12):1413.
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High-affinity binding of tumor-suppressor protein p53 and HMGB1 to hemicatenated DNA loops.
Stros M, Muselíková-Polanská E, Pospísilová S, Strauss F.
Biochemistry 2004 Jun; 43(22):7215.
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Constitutive and DNA damage inducible activation of pig3 and MDM2 genes by tumor-derived p53 mutant C277Y.
Pospisilova S, Siligan C, Ban J, Jug G, Kovar H.
Molecular Cancer Research 2004 May; 2(5):296.
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Repression of the Arf tumor suppressor by E2F3 is required for normal cell cycle kinetics.
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