Western blot using Ldb2 polyclonal antibody (Cat # PAB10273) showsdetection of a 43 KDa band corresponding to Ldb2 in a lysates prepared from human kidney (Lane 1) and mouse spleen (Lane 2) tissues. Approximately 18 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of Ldb2 polyclonal antibody. Detection occurred using a 1 : 5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hourat room temperature. A chemiluminescence system was used for signal detection using a 1 min exposure time.
Immunohistochemical staining with Ldb2 polyclonal antibody (Cat # PAB10273) was used at a 5 ug/mL to detect Ldb2 in human brain cortex tissue. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. Tissue was formalin-fixed and paraffin embedded.