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Last updated: 2017/2/19

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Ldb2 polyclonal antibody

  • Catalog # : PAB10273
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Ldb2.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 107-120 of mouse Ldb2.
  • Host:
  • Rabbit
  • Reactivity:
  • Chicken, Chimpanzee, Dog, Human, Mouse, Rat
  • Specificity:
  • This affinity purified antibody is directed against mouse LDB2.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:5000-1:20000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot using Ldb2 polyclonal antibody (Cat # PAB10273) showsdetection of a 43 KDa band corresponding to Ldb2 in a lysates prepared from human kidney (Lane 1) and mouse spleen (Lane 2) tissues.
    Approximately 18 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of Ldb2 polyclonal antibody.
    Detection occurred using a 1 : 5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hourat room temperature.
    A chemiluminescence system was used for signal detection using a 1 min exposure time.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining with Ldb2 polyclonal antibody (Cat # PAB10273) was used at a 5 ug/mL to detect Ldb2 in human brain cortex tissue. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. Tissue was formalin-fixed and paraffin embedded.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • O55203;NP_034828
  • Gene Name:
  • Ldb2
  • Gene Alias:
  • AI035351,AW146358,CLIM1,CLP-36,Ldb3
  • Gene Description:
  • LIM domain binding 2
  • Other Designations:
  • -
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