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Hif3a polyclonal antibody

  • Catalog # : PAB10271
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Hif3a.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 581-592 of mouse Hif3a.
  • Host:
  • Rabbit
  • Reactivity:
  • Mouse, Rat
  • Specificity:
  • This affinity-purified antibody is directed against mouse Hif3alpha protein. No reactivity is expected against other forms of Hif proteins.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:14000-1:80000)
    Western Blot (1:10500-1:8000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using Hif3a polyclonal antibody (Cat # PAB10271) shows detection of aband ~72 KDa corresponding to mouse Hif3a (arrowhead).
    Approximately 10 ug of a CoCl2 treated NIH/3T3 cell lysate (Lane 1) and control NIH/3T3 cell lysate (Lane 2) were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.
    Treatment of exponentially growing NIH/3T3 cells with 130 uM CoCl2 for 6 h at 37°C effectively mimicshypoxia.
    After blocking the membrane was probed overnight at 4°C with the primary antibody diluted to 1:1,600.
    The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
    IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDye is a trademark of LI-COR, Inc.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Protein Accession#:
  • Q9Z2I5;NP_058564
  • Gene Name:
  • Hif3a
  • Gene Alias:
  • Ipas,MOP7
  • Gene Description:
  • hypoxia inducible factor 3, alpha subunit
  • Other Designations:
  • OTTMUSP00000026202,hypoxia inducible factor three alpha,inhibitory PAS domain protein
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