Hif3a polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot using Hif3a polyclonal antibody (Cat # PAB10271) shows detection of aband ~72 KDa corresponding to mouse Hif3a (arrowhead).
Approximately 10 ug of a CoCl2 treated NIH/3T3 cell lysate (Lane 1) and control NIH/3T3 cell lysate (Lane 2) were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.
Treatment of exponentially growing NIH/3T3 cells with 130 uM CoCl2 for 6 h at 37°C effectively mimicshypoxia.
After blocking the membrane was probed overnight at 4°C with the primary antibody diluted to 1:1,600.
The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Hif3a.
Immunogen
A synthetic peptide corresponding to amino acids 581-592 of mouse Hif3a.
Host
Rabbit
Reactivity
Mouse, Rat
Specificity
This affinity-purified antibody is directed against mouse Hif3alpha protein. No reactivity is expected against other forms of Hif proteins.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:14000-1:80000)
Western Blot (1:10500-1:8000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot using Hif3a polyclonal antibody (Cat # PAB10271) shows detection of aband ~72 KDa corresponding to mouse Hif3a (arrowhead).
Approximately 10 ug of a CoCl2 treated NIH/3T3 cell lysate (Lane 1) and control NIH/3T3 cell lysate (Lane 2) were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.
Treatment of exponentially growing NIH/3T3 cells with 130 uM CoCl2 for 6 h at 37°C effectively mimicshypoxia.
After blocking the membrane was probed overnight at 4°C with the primary antibody diluted to 1:1,600.
The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — Hif3a
Entrez GeneID
53417Protein Accession#
Q9Z2I5;NP_058564Gene Name
Hif3a
Gene Alias
Ipas, MOP7
Gene Description
hypoxia inducible factor 3, alpha subunit
Gene Ontology
HyperlinkGene Summary
O
Other Designations
OTTMUSP00000026202|hypoxia inducible factor three alpha|inhibitory PAS domain protein
-
Publication Reference
-
Structural basis for PAS domain heterodimerization in the basic helix--loop--helix-PAS transcription factor hypoxia-inducible factor.
Erbel PJ, Card PB, Karakuzu O, Bruick RK, Gardner KH.
PNAS 2003 Dec; 100(26):15504.
-
Inhibitory PAS domain protein (IPAS) is a hypoxia-inducible splicing variant of the hypoxia-inducible factor-3alpha locus.
Makino Y, Kanopka A, Wilson WJ, Tanaka H, Poellinger L.
The Journal of Biological Chemistry 2002 Sep; 277(36):32405.
-
Cross-talk between the aryl hydrocarbon receptor and hypoxia inducible factor signaling pathways. Demonstration of competition and compensation.
Chan WK, Yao G, Gu YZ, Bradfield CA.
The Journal of Biological Chemistry 1999 Apr; 274(17):12115.
-
Structural basis for PAS domain heterodimerization in the basic helix--loop--helix-PAS transcription factor hypoxia-inducible factor.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com