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Last updated: 2016/12/4
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Gli2 polyclonal antibody

  • Catalog # : PAB10270
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Gli2.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of mouse Gli2.
  • Host:
  • Rabbit
  • Reactivity:
  • Mouse, Rat
  • Specificity:
  • This affinity purified antibody is directed against mouse Gli-2 protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:15000-1:60000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (2-20 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot using Gli2 polyclonal antibody (Cat # PAB10270) shows detection of a predominant band at ~190 KDa corresponding to Gli2 (arrowhead) in mouse brain tissue lysate (Lane 1).
    Pre-incubation of antibody with immunizing peptide completely blocks staining of this band (Lane 2).
    Approximately 25 ug of lysate was resolved on a 4-8% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose.
    After blocking with 5% goat serum and 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1 : 750.
    Incubation was at room temperature for 2 h followed by washes and reaction with a 1 : 10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature.
    Molecular weight markers are shown (M) using the 700 nm channel (red).
    IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDye is a trademark of LI-COR, Inc.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of Gli2 polyclonal antibody (Cat # PAB10270) was used at 10 ug/mL to evaluate staining on several mouse tissues.
    Moderate to strong staining was seen on many tissues, with low background staining.
    This image shows Gli2 staining of mouse testis.
    Tissue was formalin-fixed and paraffin embedded.
    Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of Gli2 polyclonal antibody (Cat # PAB10270) was used at 10 ug/mL to evaluate staining on several mouse tissues.
    Moderate to strong staining was seen on many tissues with low background staining.
    This image shows Gli2 staining of mouse brain.
    Tissue was formalin-fixed and paraffin embedded.
    Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • Q8K0K3(fragment);XP_136212
  • Gene Name:
  • Gli2
  • Gene Alias:
  • AW546128
  • Gene Description:
  • GLI-Kruppel family member GLI2
  • Other Designations:
  • Tax helper protein
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