Gli2 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot using Gli2 polyclonal antibody (Cat # PAB10270) shows detection of a predominant band at ~190 KDa corresponding to Gli2 (arrowhead) in mouse brain tissue lysate (Lane 1).
Pre-incubation of antibody with immunizing peptide completely blocks staining of this band (Lane 2).
Approximately 25 ug of lysate was resolved on a 4-8% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking with 5% goat serum and 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1 : 750.
Incubation was at room temperature for 2 h followed by washes and reaction with a 1 : 10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature.
Molecular weight markers are shown (M) using the 700 nm channel (red).
IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of Gli2 polyclonal antibody (Cat # PAB10270) was used at 10 ug/mL to evaluate staining on several mouse tissues.
Moderate to strong staining was seen on many tissues with low background staining.
This image shows Gli2 staining of mouse brain.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of Gli2 polyclonal antibody (Cat # PAB10270) was used at 10 ug/mL to evaluate staining on several mouse tissues.
Moderate to strong staining was seen on many tissues, with low background staining.
This image shows Gli2 staining of mouse testis.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Gli2.
Immunogen
A synthetic peptide corresponding to internal region of mouse Gli2.
Host
Rabbit
Reactivity
Mouse, Rat
Specificity
This affinity purified antibody is directed against mouse Gli-2 protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:15000-1:60000)
Western Blot (1:500-1:2000)
Immunohistochemistry (2-20 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot using Gli2 polyclonal antibody (Cat # PAB10270) shows detection of a predominant band at ~190 KDa corresponding to Gli2 (arrowhead) in mouse brain tissue lysate (Lane 1).
Pre-incubation of antibody with immunizing peptide completely blocks staining of this band (Lane 2).
Approximately 25 ug of lysate was resolved on a 4-8% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking with 5% goat serum and 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1 : 750.
Incubation was at room temperature for 2 h followed by washes and reaction with a 1 : 10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature.
Molecular weight markers are shown (M) using the 700 nm channel (red).
IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of Gli2 polyclonal antibody (Cat # PAB10270) was used at 10 ug/mL to evaluate staining on several mouse tissues.
Moderate to strong staining was seen on many tissues with low background staining.
This image shows Gli2 staining of mouse brain.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of Gli2 polyclonal antibody (Cat # PAB10270) was used at 10 ug/mL to evaluate staining on several mouse tissues.
Moderate to strong staining was seen on many tissues, with low background staining.
This image shows Gli2 staining of mouse testis.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Gli2
Entrez GeneID
14633Protein Accession#
Q8K0K3(fragment);XP_136212Gene Name
Gli2
Gene Alias
AW546128
Gene Description
GLI-Kruppel family member GLI2
Gene Ontology
HyperlinkOther Designations
Tax helper protein
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Publication Reference
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Gli3 null mice display glandular overgrowth of the developing stomach.
Kim JH, Huang Z, Mo R.
Developmental Dynamics 2005 Dec; 234(4):984.
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Molecular control of spinal accessory motor neuron/axon development in the mouse spinal cord.
Dillon AK, Fujita SC, Matise MP, Jarjour AA, Kennedy TE, Kollmus H, Arnold HH, Weiner JA, Sanes JR, Kaprielian Z.
The Journal of Neuroscience 2005 Nov; 25(44):10119.
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Gli2 and Gli3 have redundant and context-dependent function in skeletal muscle formation.
McDermott A, Gustafsson M, Elsam T, Hui CC, Emerson CP Jr, Borycki AG.
Development 2005 Jan; 132(2):345.
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Gli3 null mice display glandular overgrowth of the developing stomach.
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