Western blot using MLF1IP polyclonal antibody (Cat # PAB10266) shows detection of MLF1IP (arrowhead) in HeLa cells transfected with ZZ-tagged MLF1IP (lane 1). Lane 2 is lysate from non-transfected HeLa cells, and Lane 3 is lysate from HeLa cells containing a knock-out mutation for MLF1IP. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.
The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). MLF1IP, or CENPU, is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM
KSHV latent nuclear antigen interacting protein 1,centromere protein U,polo-box-interacting protein 1