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MLF1IP polyclonal antibody

  • Catalog # : PAB10266
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of MLF1IP.
  • Immunogen:
  • A synthetic peptide corresponding to residues surrounding T78 of human MLF1IP.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Chimpanzee, Dog, Human, Mouse, Rat
  • Specificity:
  • This affinity purified antibody is directed against human MLF1IP protein. This antibody reacts with MLF1IP protein that is either phosphorylated or non-phosphorylated at Thr78.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:5000-1:25000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot using MLF1IP polyclonal antibody (Cat # PAB10266) shows detection of MLF1IP (arrowhead) in HeLa cells transfected with ZZ-tagged MLF1IP (lane 1).
    Lane 2 is lysate from non-transfected HeLa cells, and Lane 3 is lysate from HeLa cells containing a knock-out mutation for MLF1IP.
    Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.
  • Immunohistochemistry
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_078905;Q71F23
  • Gene Name:
  • MLF1IP
  • Gene Alias:
  • CENP-50,CENP-U,CENP-U(50),CENP50,CENPU,FLJ23468,KLIP1,PBIP1
  • Gene Description:
  • MLF1 interacting protein
  • Gene Summary:
  • The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). MLF1IP, or CENPU, is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM
  • Other Designations:
  • KSHV latent nuclear antigen interacting protein 1,centromere protein U,polo-box-interacting protein 1
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