CRTC1 polyclonal antibody
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More Files
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Western Blot
Western blot using CRTC1 polyclonal antibody (Cat # PAB10265) shows detection of CRTC1 (lower arrowhead) and CRTC1 : MAML2 fusion protein (top arrowhead) in cell lysates.
Lane 1 contains lysate from cells expressing CRTC1 only.
Lane 2 contains lysate from cells transfected with fusion protein.
Lane 3 contains lysate from cells strongly expressing CRTC1 and weakly expressing the fusion protein.
Lane 4 contains lysate from control cells. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000.
Personal Communication, Frederick Kaye, CCR-NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CRTC1.
Immunogen
A synthetic peptide corresponding to amino acids 19-34 of human CRTC1.
Host
Rabbit
Reactivity
Bovine, Human, Mouse, Pufferfishes, Rat
Specificity
This affinity-purified antibody is directed against human MECT1 protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:40000)
Western Blot (1:500-1:20000
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot using CRTC1 polyclonal antibody (Cat # PAB10265) shows detection of CRTC1 (lower arrowhead) and CRTC1 : MAML2 fusion protein (top arrowhead) in cell lysates.
Lane 1 contains lysate from cells expressing CRTC1 only.
Lane 2 contains lysate from cells transfected with fusion protein.
Lane 3 contains lysate from cells strongly expressing CRTC1 and weakly expressing the fusion protein.
Lane 4 contains lysate from control cells. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000.
Personal Communication, Frederick Kaye, CCR-NCI, Bethesda, MD.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CRTC1
Entrez GeneID
23373Protein Accession#
NP_056136(isoform1);Q6UUV9Gene Name
CRTC1
Gene Alias
FLJ14027, KIAA0616, MECT1, TORC1, WAMTP1
Gene Description
CREB regulated transcription coactivator 1
Omim ID
607536Gene Ontology
HyperlinkOther Designations
mucoepidermoid carcinoma translocated 1|transducer of regulated cAMP response element-binding protein (CREB) 1
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Interactome
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Disease
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Publication Reference
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Mect1-Maml2 fusion oncogene linked to the aberrant activation of cyclic AMP/CREB regulated genes.
Coxon A, Rozenblum E, Park YS, Joshi N, Tsurutani J, Dennis PA, Kirsch IR, Kaye FJ.
Cancer Research 2005 Aug; 65(16):7137.
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TORCs: transducers of regulated CREB activity.
Conkright MD, Canettieri G, Screaton R, Guzman E, Miraglia L, Hogenesch JB, Montminy M.
Molecular Cell 2003 Aug; 12(2):413.
Application：IP, Human, HEK 293T cells.
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t(11;19)(q21;p13) translocation in mucoepidermoid carcinoma creates a novel fusion product that disrupts a Notch signaling pathway.
Tonon G, Modi S, Wu L, Kubo A, Coxon AB, Komiya T, O'Neil K, Stover K, El-Naggar A, Griffin JD, Kirsch IR, Kaye FJ.
Nature Genetics 2003 Feb; 33(2):208.
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Mect1-Maml2 fusion oncogene linked to the aberrant activation of cyclic AMP/CREB regulated genes.
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