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Last updated: 2016/12/4
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CRTC1 polyclonal antibody

  • Catalog # : PAB10265
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of CRTC1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 19-34 of human CRTC1.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Human, Mouse, Pufferfishes, Rat
  • Specificity:
  • This affinity-purified antibody is directed against human MECT1 protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:10000-1:40000)
    Western Blot (1:500-1:20000
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western blot using CRTC1 polyclonal antibody (Cat # PAB10265) shows detection of CRTC1 (lower arrowhead) and CRTC1 : MAML2 fusion protein (top arrowhead) in cell lysates.
    Lane 1 contains lysate from cells expressing CRTC1 only.
    Lane 2 contains lysate from cells transfected with fusion protein.
    Lane 3 contains lysate from cells strongly expressing CRTC1 and weakly expressing the fusion protein.
    Lane 4 contains lysate from control cells. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000.
    Personal Communication, Frederick Kaye, CCR-NCI, Bethesda, MD.
  • ELISA
  • Application Image
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_056136(isoform1);Q6UUV9
  • Gene Name:
  • CRTC1
  • Gene Alias:
  • FLJ14027,KIAA0616,MECT1,TORC1,WAMTP1
  • Gene Description:
  • CREB regulated transcription coactivator 1
  • Other Designations:
  • mucoepidermoid carcinoma translocated 1,transducer of regulated cAMP response element-binding protein (CREB) 1
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