Rabbit polyclonal antibody raised against synthetic peptide of MCM2.
A synthetic peptide corresponding to amino acids 21-31 of human MCM2.
Human, Mouse, Rat, Yeast
This affinity-purified antibody is directed against human MCM2 protein. Approximately equivalent reactivity occurs against both unphosphorylated and phosphorylated forms of human MCM2 (phosphorylated at residues pS26 and pS27).
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
ELISA (1:10000-1:50000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot using MCM2 polyclonal antibody (Cat # PAB10264) shows detection of both phosphorylated and unphosphorylated MCM2 presentin nuclear extracts from elutriated human cells (MO59K/K-562). The MCM2 protein is phosphorylated after initiation of DNA replication, therefore, the proteinis unphosphorylated in early S phase, and gradually becomes phosphorylated throughout S phase. In G2/M, all MCM2 is phosphorylated. Panel A shows western blot results for lysates were prepared from asynchronous cells (Lane 1), cells arrested in early S with aphidicolin (lane2), and cells arrested in mitosis with nocodazole (Lane 3). Panel B shows a schematic diagram of bands representing phosphorylated and unphosphorylated MCM2 present in these preparations. Asynchronous cells contain a doublet of both forms. Aphidicolin treated cells contain only unphosphorylated MCM2 and nocodozole treatment results in only phosphorylated MCM2 detected in the lysate. The phosphorylated band migr
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. [provided by RefSeq
DNA replication licensing factor MCM2,MCM2 minichromosome maintenance deficient 2, mitotin,cell devision cycle-like 1,cyclin-like 1,minichromosome maintenance deficient 2 (mitotin),nuclear protein BM28