MAPKAP Kinase 2 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Affinity purified MAPKAP Kinase 2 polyclonal antibody (Cat # PAB10263) detects MAPKAP Kinase 2 in unstimulated human HeLa whole cell lysate by western blot.
MAPKAP Kinase 2 polyclonal antibody used at a 1 : 2000 dilution to detect 20 ug of whole cell lysate containing MAPKAP Kinase 2.
This antibody detects a single 44 KDa protein as indicated in crude extracts prepared from either unstimulatedor TNFalpha stimulated human HeLa cell lysates.
A 4-20% gradient gel was used to separate the protein by SDS-PAGE.
The protein was transferred to nitrocellulose using standard methods.
After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1 : 5,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature.
LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAPKAP Kinase 2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 310-325 of rabbit MAPKAP Kinase 2.
Sequence
MTSALATMRVDYEQIK
Host
Rabbit
Reactivity
Chicken, Clawed frog, Hamster, Human, Mouse, Rabbit, Rat
Specificity
This polyclonal anti-MAPKAP Kinase 2 antibody recognizes native and over-expressed protein found in various tissues and extracts.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:15000)
Western Blot (1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Affinity purified MAPKAP Kinase 2 polyclonal antibody (Cat # PAB10263) detects MAPKAP Kinase 2 in unstimulated human HeLa whole cell lysate by western blot.
MAPKAP Kinase 2 polyclonal antibody used at a 1 : 2000 dilution to detect 20 ug of whole cell lysate containing MAPKAP Kinase 2.
This antibody detects a single 44 KDa protein as indicated in crude extracts prepared from either unstimulatedor TNFalpha stimulated human HeLa cell lysates.
A 4-20% gradient gel was used to separate the protein by SDS-PAGE.
The protein was transferred to nitrocellulose using standard methods.
After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1 : 5,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature.
LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Retaining of the assembly capability of vimentin phosphorylated by mitogen-activated protein kinase-activated protein kinase-2.
Cheng TJ, Tseng YF, Chang WM, Chang MD, Lai YK.
Journal of Cellular Biochemistry 2003 Jun; 89(3):589.
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Inhibition of SAPK2a/p38 prevents hnRNP A0 phosphorylation by MAPKAP-K2 and its interaction with cytokine mRNAs.
Rousseau S, Morrice N, Peggie M, Campbell DG, Gaestel M, Cohen P.
The EMBO Journal 2002 Dec; 21(23):6505.
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MAPKAP kinase 2 is essential for LPS-induced TNF-alpha biosynthesis.
Kotlyarov A, Neininger A, Schubert C, Eckert R, Birchmeier C, Volk HD, Gaestel M.
Nature Cell Biology 1999 Jun; 1(2):94.
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Retaining of the assembly capability of vimentin phosphorylated by mitogen-activated protein kinase-activated protein kinase-2.
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