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MAPKAP Kinase 2 polyclonal antibody

  • Catalog # : PAB10263
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of MAPKAP Kinase 2.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to amino acids 310-325 of rabbit MAPKAP Kinase 2.
  • Sequence:
  • MTSALATMRVDYEQIK
  • Host:
  • Rabbit
  • Reactivity:
  • Chicken, Clawed frog, Hamster, Human, Mouse, Rabbit, Rat
  • Specificity:
  • This polyclonal anti-MAPKAP Kinase 2 antibody recognizes native and over-expressed protein found in various tissues and extracts.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:15000)
    Western Blot (1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Affinity purified MAPKAP Kinase 2 polyclonal antibody (Cat # PAB10263) detects MAPKAP Kinase 2 in unstimulated human HeLa whole cell lysate by western blot.
    MAPKAP Kinase 2 polyclonal antibody used at a 1 : 2000 dilution to detect 20 ug of whole cell lysate containing MAPKAP Kinase 2.
    This antibody detects a single 44 KDa protein as indicated in crude extracts prepared from either unstimulatedor TNFalpha stimulated human HeLa cell lysates.
    A 4-20% gradient gel was used to separate the protein by SDS-PAGE.
    The protein was transferred to nitrocellulose using standard methods.
    After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1 : 5,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature.
    LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
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