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MFAP5 polyclonal antibody

  • Catalog # : PAB10261
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of MFAP5.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human MFAP5.
  • Host:
  • Rabbit
  • Reactivity:
  • Chimpanzee, Dog, Human, Mouse, Rat
  • Specificity:
  • This affinity-purified antibody is directed against human MAGP-2 protein
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:5000-1:20000)
    Western Blot (1:250-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot using MFAP5 polyclonal antibody (Cat # PAB10261) shows detection (arrowhead) of secreted MFAP5 (lane 2) and MFAP5 present in a MFAP5 transfected HEK293 lysate (lane 3).
    No staining is detected in supernatants from non-transfected cells (lane 1).
    After SDS-PAGE and transfer the membrane was probed with the primary antibody diluted to 1:100 in TBST with 5% BSA overnight at 4°C.
    Personal Communication, Micheal Birrer, CCR-NCI, Bethesda, MD.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 8076
  • Gene Name:
  • MFAP5
  • Gene Alias:
  • MAGP2,MP25
  • Gene Description:
  • microfibrillar associated protein 5
  • Gene Summary:
  • This gene encodes a 25-kD microfibril-associated glycoprotein which is rich in serine and threonine residues. It lacks a hydrophobic carboxyl terminus and proline-, glutamine-, and tyrosine-rich regions, which are characteristics of a related 31-kDa microfibril-associated glycoprotein (MFAP2). The close similarity between these two proteins is confined to a central region of 60 aa where precise alignment of 7 cysteine residues occurs. The structural differences suggest that this encoded protein has some functions that are distinct from those of MFAP2. [provided by RefSeq
  • Other Designations:
  • microfibril-associated glycoprotein 2,microfibril-associated glycoprotein-2
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