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Last updated: 2016/11/27
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LIMK1 polyclonal antibody

  • Catalog # : PAB10258
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of LIMK1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 630-647 of human LIMK1.
  • Host:
  • Rabbit
  • Reactivity:
  • Chimpanzee, Human, Mouse, Orangutan, Rat
  • Specificity:
  • This affinity purified antibody is directed against human LIM kinase protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:8000-1:36000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot using LIMK1 polyclonal antibody (Cat # PAB10258) shows detection of a 73 KDa band corresponding to LIMK1 in lysates from mouse brain.
    Approximately 18 ug of lysate wasrun on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of LIMK1 polyclonal antibody.
    The doublet band at ~ 75 KDa may represent phosphorylated and non-phosphorylated forms of the protein.
    The identity of the strong lower molecular weight band at approximately 20 KDa is unknown.
    Signal was detected using standard techniques.
  • Immunofluorescence
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunofluorescence
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 3984
  • Protein Accession#:
  • P53667;NP_002305(isoform1)
  • Gene Name:
  • LIMK1
  • Gene Alias:
  • LIMK
  • Gene Description:
  • LIM domain kinase 1
  • Gene Summary:
  • There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. [provided by RefSeq
  • Other Designations:
  • LIM motif-containing protein kinase,OTTHUMP00000025066
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