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Ldb1 polyclonal antibody

  • Catalog # : PAB10257
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Ldb1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 361-373 of mouse Ldb1.
  • Host:
  • Rabbit
  • Reactivity:
  • Chicken, Chimpanzee, Dog, Frog, Human, Mouse, Rat
  • Specificity:
  • This affinity purified antibody is directed against the mouse LDB1protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:170000)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using Ldb1 polyclonal antibody (Cat # PAB10257) shows detection of Ldb1 protein (arrowhead) in Jurkat whole cell lysate.
    Approximately 30 ug of lysate was loaded prior to separation and transfer to nitrocellulose.
    Primary antibody was used at a 1 : 1,800 dilution in 5% BLOTTO in PBS reacted overnight at 4°C.
    The membrane was washed and reacted with a 1:20,000 dilution of DyLight™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green).
    Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
    Fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDye is a trademark of LI-COR, Inc.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • P70662;NP_034827
  • Gene Name:
  • Ldb1
  • Gene Alias:
  • CLIM2,NLI
  • Gene Description:
  • LIM domain binding 1
  • Other Designations:
  • carboxyl-terminal LIM domain-binding protein 2
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