Western blot using Ldb1 polyclonal antibody (Cat # PAB10257) shows detection of Ldb1 protein (arrowhead) in Jurkat whole cell lysate. Approximately 30 ug of lysate was loaded prior to separation and transfer to nitrocellulose. Primary antibody was used at a 1 : 1,800 dilution in 5% BLOTTO in PBS reacted overnight at 4°C. The membrane was washed and reacted with a 1:20,000 dilution of DyLight™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). Fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.