Ldb1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using Ldb1 polyclonal antibody (Cat # PAB10257) shows detection of Ldb1 protein (arrowhead) in Jurkat whole cell lysate.
Approximately 30 ug of lysate was loaded prior to separation and transfer to nitrocellulose.
Primary antibody was used at a 1 : 1,800 dilution in 5% BLOTTO in PBS reacted overnight at 4°C.
The membrane was washed and reacted with a 1:20,000 dilution of DyLight™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
Fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Ldb1.
Immunogen
A synthetic peptide corresponding to amino acids 361-373 of mouse Ldb1.
Host
Rabbit
Reactivity
Chicken, Chimpanzee, Dog, Frog, Human, Mouse, Rat
Specificity
This affinity purified antibody is directed against the mouse LDB1protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:170000)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using Ldb1 polyclonal antibody (Cat # PAB10257) shows detection of Ldb1 protein (arrowhead) in Jurkat whole cell lysate.
Approximately 30 ug of lysate was loaded prior to separation and transfer to nitrocellulose.
Primary antibody was used at a 1 : 1,800 dilution in 5% BLOTTO in PBS reacted overnight at 4°C.
The membrane was washed and reacted with a 1:20,000 dilution of DyLight™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
Fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Ldb1
Entrez GeneID
16825Protein Accession#
P70662;NP_034827Gene Name
Ldb1
Gene Alias
CLIM2, NLI
Gene Description
LIM domain binding 1
Gene Ontology
HyperlinkOther Designations
carboxyl-terminal LIM domain-binding protein 2
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Publication Reference
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Identification of a TAL1 target gene reveals a positive role for the LIM domain-binding protein Ldb1 in erythroid gene expression and differentiation.
Xu Z, Huang S, Chang LS, Agulnick AD, Brandt SJ.
Molecular and Cellular Biology 2003 Nov; 23(21):7585.
Application：ChIP, Func, Mouse, MEL cells.
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Functional ablation of the mouse Ldb1 gene results in severe patterning defects during gastrulation.
Mukhopadhyay M, Teufel A, Yamashita T, Agulnick AD, Chen L, Downs KM, Schindler A, Grinberg A, Huang SP, Dorward D, Westphal H.
Development 2003 Feb; 130(3):495.
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Genomic structure and chromosomal localization of the mouse LIM domain-binding protein 1 gene, Ldb1.
Yamashita T, Agulnick AD, Copeland NG, Gilbert DJ, Jenkins NA, Westphal H.
Genomics 1998 Feb; 48(1):87.
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Identification of a TAL1 target gene reveals a positive role for the LIM domain-binding protein Ldb1 in erythroid gene expression and differentiation.
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