Rabbit polyclonal antibody raised against full length recombinant lacI.
Recombinant protein corresponding to full length Escherichia coli (strain DH10B) lacI.
This antibody reacts with recombinant Lac I protein. A BLAST analysis was used to suggest cross-reactivity with Lac I from DNA-binding transcriptional repressor in Escherichia coli (strain DH10B) and transcriptional repressor of the lac operon in Shigella sonnei (strain Ss046) based on a 100% homology with the immunizing sequence.
Quality Control Testing:
Antibody Reactive Against Recombinant Protein.
ELISA (1:90000-1:100000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot using lacI polyclonal antibody (Cat # PAB10255) shows detection of a 38 kDa band corresponding to recombinant lacI (arrowhead). The blot was blocked with 5% BLOTTO in TTBS overnight at 4°C. Primary antibody was used at a 1 : 1,000 dilution followed by reaction with a 1 : 10,000 dilution of HRP goat anti-rabbit IgG. ECL was used for detection. Personal communication, S. Hughes & M. Abram, NCI, Bethesda, MD.