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Last updated: 2016/11/27
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lacI polyclonal antibody

  • Catalog # : PAB10255
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against full length recombinant lacI.
  • Immunogen:
  • Recombinant protein corresponding to full length Escherichia coli (strain DH10B) lacI.
  • Host:
  • Rabbit
  • Specificity:
  • This antibody reacts with recombinant Lac I protein. A BLAST analysis was used to suggest cross-reactivity with Lac I from DNA-binding transcriptional repressor in Escherichia coli (strain DH10B) and transcriptional repressor of the lac operon in Shigella sonnei (strain Ss046) based on a 100% homology with the immunizing sequence.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:90000-1:100000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot using lacI polyclonal antibody (Cat # PAB10255) shows detection of a 38 kDa band corresponding to recombinant lacI (arrowhead). The blot was blocked with 5% BLOTTO in TTBS overnight at 4°C. Primary antibody was used at a 1 : 1,000 dilution followed by reaction with a 1 : 10,000 dilution of HRP goat anti-rabbit IgG. ECL was used for detection.
    Personal communication, S. Hughes & M. Abram, NCI, Bethesda, MD.
  • ELISA
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • AAA24052;P03023
  • Gene Name:
  • lacI
  • Gene Alias:
  • ECK0342,JW0336
  • Gene Description:
  • DNA-binding transcriptional repressor
  • Other Designations:
  • -
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