HHV8gp59 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot using HHV8gp59 polyclonal antibody (Cat # PAB10254) to detect HHV8gp59 in HEK293 cells transfected with HHV8gp59 expression vector and HHV8gp59 truncations, or in HHV8gp59 infected B-cell line (BCBL-1) treated with or without valproic acid to induce viral replication (arrow).
The membrane was probed with the primary antibody diluted 1 : 7,500 (left) and 1:1,000 (right).
Personal Communication, V. Majerciak, M.Zheng, CCR-NC , Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HHV8gp59.
Immunogen
A synthetic peptide corresponding to C-terminus of human herpesvirus 8 type M HHV8gp59.
Host
Rabbit
Reactivity
Viruses
Specificity
A BLAST analysis was used to suggest cross-reactivity with ORF57 protein from human herpesvirus 8 types P and M sources based on 94% homology with the immunizing sequence.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:100000-1:170000)
Western Blot (1:5000-1:8000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using HHV8gp59 polyclonal antibody (Cat # PAB10254) to detect HHV8gp59 in HEK293 cells transfected with HHV8gp59 expression vector and HHV8gp59 truncations, or in HHV8gp59 infected B-cell line (BCBL-1) treated with or without valproic acid to induce viral replication (arrow).
The membrane was probed with the primary antibody diluted 1 : 7,500 (left) and 1:1,000 (right).
Personal Communication, V. Majerciak, M.Zheng, CCR-NC , Bethesda, MD.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — HHV8gp59
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Publication Reference
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Targeted disruption of Kaposi's sarcoma-associated herpesvirus ORF57 in the viral genome is detrimental for the expression of ORF59, K8alpha, and K8.1 and the production of infectious virus.
Majerciak V, Pripuzova N, McCoy JP, Gao SJ, Zheng ZM.
Journal of Virology 2007 Feb; 81(3):1062.
Application：IF, Human, Bac36 cells.
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Structural and functional analyses of Kaposi sarcoma-associated herpesvirus ORF57 nuclear localization signals in living cells.
Majerciak V, Yamanegi K, Nie SH, Zheng ZM.
The Journal of Biological Chemistry 2006 Sep; 281(38):28365.
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The evolutionarily conserved Kaposi's sarcoma-associated herpesvirus ORF57 protein interacts with REF protein and acts as an RNA export factor.
Malik P, Blackbourn DJ, Clements JB.
The Journal of Biological Chemistry 2004 Jul; 279(31):33001.
Application：IP, WB, Human, BCBL-1 cells.
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Targeted disruption of Kaposi's sarcoma-associated herpesvirus ORF57 in the viral genome is detrimental for the expression of ORF59, K8alpha, and K8.1 and the production of infectious virus.
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