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HHV8gp59 polyclonal antibody

  • Catalog # : PAB10254
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of HHV8gp59.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human herpesvirus 8 type M HHV8gp59.
  • Host:
  • Rabbit
  • Reactivity:
  • Viruses
  • Specificity:
  • A BLAST analysis was used to suggest cross-reactivity with ORF57 protein from human herpesvirus 8 types P and M sources based on 94% homology with the immunizing sequence.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:100000-1:170000)
    Western Blot (1:5000-1:8000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot using HHV8gp59 polyclonal antibody (Cat # PAB10254) to detect HHV8gp59 in HEK293 cells transfected with HHV8gp59 expression vector and HHV8gp59 truncations, or in HHV8gp59 infected B-cell line (BCBL-1) treated with or without valproic acid to induce viral replication (arrow).
    The membrane was probed with the primary antibody diluted 1 : 7,500 (left) and 1:1,000 (right).
    Personal Communication, V. Majerciak, M.Zheng, CCR-NC , Bethesda, MD.
  • Immunohistochemistry
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry
  • ELISA
  • Gene Information
  • Gene Name:
  • HHV8gp59
  • Gene Alias:
  • -
  • Gene Description:
  • similar to immediate early protein; similar to EBV BMLF1
  • Other Designations:
  • ORF 57
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